Trans-cleaving hammerhead ribozyme in specific regions can improve knockdown efficiency in vivo.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of cellular biochemistry"
DOI: 10.1002/jcb.30249
Abstract: Trans-cleaving techniques have been most enthusiastically embraced in the development of therapy for genetic diseases, particularly in the correction of monogenic recessive mutations at the messenger RNA level. However, easy degradation and poor catalytic activity… read more here.
Keywords: trans cleaving; ribozyme specific; cleaving hammerhead; hammerhead ribozyme ... See more keywords
Structural Simplicity and Mechanistic Complexity in the Hammerhead Ribozyme.
Sign Up to like & getrecommendations! Published in 2018 at "Progress in molecular biology and translational science"
DOI: 10.1016/bs.pmbts.2018.07.006
Abstract: Natural or full-length hammerhead ribozymes are up to 1000-fold more active than their minimal counterparts that lack a complex tertiary interaction that pre-organizes and stabilizes the ribozyme active site, positioning RNA functional groups to facilitate… read more here.
Keywords: hammerhead; hammerhead ribozyme; mechanistic complexity; structural simplicity ... See more keywords
Dynamic nuclear polarization on a hybridized hammerhead ribozyme: An explorative study of RNA folding and direct DNP with a paramagnetic metal ion cofactor.
Sign Up to like & getrecommendations! Published in 2019 at "Solid state nuclear magnetic resonance"
DOI: 10.1016/j.ssnmr.2019.04.005
Abstract: While uniform isotope labeling of ribonucleic acids (RNA) can simply and efficiently be achieved by in-vitro transcription, the specific introduction of nucleotides in larger constructs is non-trivial and often ineffective. Here, we demonstrate how a… read more here.
Keywords: hammerhead ribozyme; dnp; ribozyme; nuclear polarization ... See more keywords