Generation of High Expressing Chinese Hamster Ovary Cell Pools Using the Leap-In Transposon System.
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology journal"
DOI: 10.1002/biot.201700748
Abstract: Clonally derived cell lines (CDCL) from Chinese Hamster Ovary (CHO) host cell lines, remain the most popular method to manufacture therapeutic proteins. However, CHO cell pools are increasingly being used as an alternate method to… read more here.
Keywords: cell pools; hamster ovary; chinese hamster; generation high ... See more keywords
Untangling the mechanism of 3‐methyladenine in enhancing the specific productivity: Transcriptome analysis of recombinant Chinese hamster ovary cells treated with 3‐methyladenine
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26777
Abstract: 3‐Methyladenine (3‐MA) is a chemical additive that enhances the specific productivity (q p) in recombinant Chinese hamster ovary (rCHO) cell lines. Different from its widely known function of inhibiting autophagy, 3‐MA has instead shown to… read more here.
Keywords: mechanism; specific productivity; hamster ovary; recombinant chinese ... See more keywords
Constitutively active Rheb mutants [T23M] and [E40K] drive increased production and secretion of recombinant protein in Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27748
Abstract: Monoclonal antibodies (mAbs) are high value agents used for disease therapy (“biologic drugs”) or as diagnostic tools which are widely used in the healthcare sector. They are generally manufactured in mammalian cells, in particular Chinese… read more here.
Keywords: protein; constitutively active; rheb; chinese hamster ... See more keywords
Extracellular vesicles facilitate large‐scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28053
Abstract: Cells in culture are viewed as unique individuals in a large population communicating through extracellular molecules and, more recently extracellular vesicles (EVs). Our data here paint a different picture: large‐scale exchange of cellular material through… read more here.
Keywords: extracellular vesicles; chinese hamster; hamster ovary; large scale ... See more keywords
SLAM‐seq reveals early transcriptomic response mechanisms upon glutamine deprivation in Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28320
Abstract: Mammalian cells frequently encounter subtle perturbations during recombinant protein production. Identifying the genetic factors that govern the cellular stress response can facilitate targeted genetic engineering to obtain production cell lines that demonstrate a higher stress… read more here.
Keywords: chinese hamster; seq; hamster ovary; slam seq ... See more keywords
Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28367
Abstract: The production of high‐value biopharmaceuticals is dominated by mammalian production cells, particularly Chinese hamster ovary (CHO) cells, which have been widely used and preferred in manufacturing processes. The discovery of CRISPR‐Cas9 significantly accelerated cell line… read more here.
Keywords: genome editing; crispr toolbox; crispr; chinese hamster ... See more keywords
Effective and efficient characterization of Chinese hamster ovary production cell lines using automated intracellular staining and statistical modeling
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2667
Abstract: Mammalian cell line development is critical to bioproduct manufacturing. Success requires selecting a line with desirable performance characteristics, including consistent expression throughout the proposed manufacturing window. Given the genetic and phenotypic flux inherent to immortalized… read more here.
Keywords: production cell; hamster ovary; chinese hamster; automated intracellular ... See more keywords
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3268
Abstract: Charge variants represent a critical quality attribute that must be controlled during the development and manufacturing of monoclonal antibodies (mAb). Previously, we reported the development of a cost‐effective enzymatic treatment capable of removing the C‐terminal… read more here.
Keywords: enzymatic treatment; cell; chinese hamster; hamster ovary ... See more keywords
Modulating and optimizing Pluronic F-68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3340
Abstract: Perfusion culture is often performed with micro-sparger to fulfill the high oxygen demand from the densified cells. Protective additive Pluronic F-68 (PF-68) is widely used to mitigate the adverse effect in cell viability from micro-sparging.… read more here.
Keywords: cell; chinese hamster; hamster ovary; cell cultures ... See more keywords
Novel short synthetic matrix attachment region for enhancing transgenic expression in recombinant Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.29165
Abstract: Matrix attachment regions (MARs) are DNA fragments with specific motifs that enhance transgenic expression; however, the characteristics and functions of these elements remain unclear. In this study, we designed and synthesized three short chimeric MARs,… read more here.
Keywords: transgenic expression; hamster ovary; chinese hamster; expression ... See more keywords
Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6972-2_3
Abstract: We describe a one-liter transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells using polyethyleneimine (PEI) for DNA delivery. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of… read more here.
Keywords: large scale; transfection; suspension; hamster ovary ... See more keywords