An effective double gene knock‐in strategy using small‐molecule L755507 in the medaka fish (Oryzias latipes)
Sign Up to like & getrecommendations! Published in 2022 at "genesis"
DOI: 10.1002/dvg.23465
Abstract: Homology‐directed repair (HDR)‐mediated genome editing has become a powerful method for altering chromosomal sequences in a seamless and accurate manner. However, the low efficiency of HDR in most cells hinders the establishment of desired strains… read more here.
Keywords: knock; small molecule; hdr mediated; molecule l755507 ... See more keywords
Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39142-0
Abstract: Editing individual nucleotides is a crucial component for validating genomic disease association. It is currently hampered by CRISPR-Cas-mediated “base editing” being limited to certain nucleotide changes, and only achievable within a small window around CRISPR-Cas… read more here.
Keywords: hdr mediated; machine learning; using machine; hdr ... See more keywords