SEC‐SAXS and HDX‐MS: A powerful combination. The case of the calcium‐binding domain of a bacterial toxin
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1577
Abstract: Small‐angle X‐ray scattering (SAXS) is a relatively simple experimental technique that provides information on the global conformation of macromolecules in solution, be they fully structured, partially, or extensively unfolded. Size exclusion chromatography in line with… read more here.
Keywords: combination; case calcium; calcium binding; saxs ... See more keywords
Online Hydrogen-Deuterium Exchange Traveling Wave Ion Mobility Mass Spectrometry (HDX-IM-MS): a Systematic Evaluation
Sign Up to like & getrecommendations! Published in 2017 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1007/s13361-017-1633-z
Abstract: AbstractHydrogen-deuterium exchange mass spectrometry (HDX-MS) is an important tool for measuring and monitoring protein structure. A bottom-up approach to HDX-MS provides peptide level deuterium uptake values and a more refined localization of deuterium incorporation compared… read more here.
Keywords: mass spectrometry; deuterium; ion mobility; hdx ... See more keywords
Quantitative Evaluation of Native Protein Folds and Assemblies by Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS)
Sign Up to like & getrecommendations! Published in 2018 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2070-3
Abstract: AbstractHydrogen deuterium exchange mass spectrometry (HDX-MS) has significant potential for protein structure initiatives but its relationship with protein conformations is unclear. We report on the efficacy of HDX-MS to distinguish between native and non-native proteins… read more here.
Keywords: protein; hdx; mass spectrometry; native protein ... See more keywords
Monitoring of the functional properties and unfolding change of Ovalbumin after DHPM treatment by HDX and FTICR MS: Functionality and unfolding of Oval after DHPM by HDX and FTICR MS.
Sign Up to like & getrecommendations! Published in 2017 at "Food chemistry"
DOI: 10.1016/j.foodchem.2017.01.109
Abstract: Dynamic high-pressure microfluidizaiton (DHPM) induced unfolding of proteins and enzymes could enhance the functional properties and hydrolytic ability of the molecules. In this study, the effect of DHPM on the surface activities of Ovalbumin (Oval),… read more here.
Keywords: functional properties; foaming emulsifying; dhpm; emulsifying properties ... See more keywords
Elucidating Protein-Ligand Interactions in Cell Lysates Using High-Throughput Hydrogen-Deuterium Exchange Mass Spectrometry with Integrated Protein Thermal Depletion.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04266
Abstract: Hydrogen-deuterium exchange coupled with mass spectrometry (HDX-MS) is a powerful technique for the characterization of protein-ligand interactions. Currently, there is a growing need for breakthroughs in the application of HDX-MS analysis to protein-ligand interactions in… read more here.
Keywords: hdx; high throughput; ligand interactions; cell lysates ... See more keywords
Cyclic Ion Mobility for Hydrogen/Deuterium Exchange-Mass Spectrometry Applications
Sign Up to like & getrecommendations! Published in 2024 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.3c05753
Abstract: Hydrogen/deuterium exchange-mass spectrometry (HDX-MS) has emerged as a powerful tool to probe protein dynamics. As a bottom-up technique, HDX-MS provides information at peptide-level resolution, allowing structural localization of dynamic changes. Consequently, the HDX-MS data quality… read more here.
Keywords: hdx; deuterium exchange; hydrogen deuterium; ion ... See more keywords
Epitope Mapping by HDX-MS Elucidates the Surface Coverage of Antigens Associated with High Blocking Efficiency of Antibodies to Birch Pollen Allergen.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01864
Abstract: Epitopes of a native pollen allergen protein, birch Bet v1, against four of the noncompeting anti-Bet v1 antibodies individually or in combination, were identified by solution-phase amide backbone H/D exchange (HDX) coupled with high-resolution Q-TOF… read more here.
Keywords: pollen allergen; blocking efficiency; anti bet; coverage ... See more keywords
HDX-MS Analysis of Catalytic Activation of IKK2 in the IκB Kinase Complex.
Sign Up to like & getrecommendations! Published in 2024 at "Biochemistry"
DOI: 10.1021/acs.biochem.4c00202
Abstract: The IκB Kinase (IKK) complex, containing catalytic IKK2 and noncatalytic NEMO subunits, plays essential roles in the induction of transcription factors of the NF-κB family. Catalytic activation of IKK2 via phosphorylation of its activation loop… read more here.
Keywords: catalytic activation; hdx; activation; activation ikk2 ... See more keywords
Practical Tips for the Application of HDX-MS to Membrane Proteins, Biomolecular Condensates, and Weak Protein Binders.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.5c00067
Abstract: Hydrogen-deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful biophysical tool to study protein thermodynamics, dynamics, and function. Despite its increasing use, effective implementation of HDX-MS requires knowledge of protein chemistry, folding, liquid chromatography,… read more here.
Keywords: condensates weak; proteins biomolecular; hdx; biomolecular condensates ... See more keywords
Investigating how intrinsically disordered regions contribute to protein function using HDX-MS.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical Society transactions"
DOI: 10.1042/bst20220206
Abstract: A large amount of the human proteome is composed of highly dynamic regions that do not adopt a single static conformation. These regions are defined as intrinsically disordered, and they are found in a third… read more here.
Keywords: hdx; intrinsically disordered; disordered regions; regions contribute ... See more keywords
Fundamentals of HDX-MS
Sign Up to like & getrecommendations! Published in 2022 at "Essays in Biochemistry"
DOI: 10.1042/ebc20220111
Abstract: Abstract Hydrogen deuterium exchange mass spectrometry (HDX-MS) is becoming part of the standard repertoire of techniques used by molecular biologists to investigate protein structure and dynamics. This is partly due to the increased use of… read more here.
Keywords: hdx; fundamentals hdx; biochemistry; microscopy ... See more keywords