Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202200062
Abstract: Expression of recombinant genes in HEK293 cells is frequently utilized for production of recombinant proteins and viral vectors. These systems frequently employ the cytomegalovirus (CMV) promoter to drive recombinant gene transcription. However, the mechanistic basis… read more here.
Keywords: cmv; expression recombinant; recombinant genes; hek293 cells ... See more keywords
Enhancing the production of adeno‐associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design‐of‐experiment optimization of triple plasmid ratio
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202300667
Abstract: The recombinant adeno‐associated virus (rAAV) vectors used in gene therapy are usually produced by transfecting three different plasmids (Adenoviral helper plasmid (pHelper), AAV rep/cap plasmids (pRepCap), and Transgene plasmid (pAAV‐GOI)) into human embryonic kidney 293… read more here.
Keywords: capsid; hek293 cells; plasmid ratio; plasmid ... See more keywords
Unraveling Cytotoxicity in HEK293 Cells During Recombinant AAV Production for Gene Therapy Applications
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Journal"
DOI: 10.1002/biot.202400501
Abstract: Transient transfection of HEK293 cells represents the dominant technique for the production of recombinant adeno‐associated virus (AAV) vectors. However, recombinant AAV (rAAV) production is cytotoxic, potentially impacting process performance, product yields, and quality, complicating downstream… read more here.
Keywords: cytotoxicity; hek293 cells; hek293; death ... See more keywords
Multivariate data analysis on multisensor measurement for inline process monitoring of adenovirus production in HEK293 cells
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28712
Abstract: In the era of Biopharma 4.0, process digitalization fundamentally requires accurate and timely monitoring of critical process parameters (CPPs) and quality attributes. Bioreactor systems are equipped with a variety of sensors to ensure process robustness… read more here.
Keywords: process; hek293 cells; adenovirus production; production hek293 ... See more keywords
HEK293 cells exposed to microcystin‐LR show reduced protein phosphatase 2A activity and more stable cytoskeletal structure when overexpressing α4 protein
Sign Up to like & getrecommendations! Published in 2017 at "Environmental Toxicology"
DOI: 10.1002/tox.22230
Abstract: Microcystin‐LR (MC‐LR) is one of the most toxic members of microcystins released by freshwater cyanobacterial. The major mechanism of MC‐LR toxicity has been attributed to its inhibition of protein phosphatases 1 (PP1) and 2A (PP2A).… read more here.
Keywords: pp2a; cells exposed; microcystin; hek293 cells ... See more keywords
Heterologous Expression and Patch-Clamp Recording of P2X Receptors in HEK293 Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_19
Abstract: P2X receptors (P2XRs) are ligand-gated ion channels gated by extracellular adenosine 5'-triphosphate (ATP) and play a critical role in mediating ATP-induced purinergic signaling in physiological and pathological processes. Heterologous expression of P2XR in human embryonic… read more here.
Keywords: p2x receptors; patch clamp; hek293 cells; clamp recording ... See more keywords
Cyclocreatine Transport by SLC6A8, the Creatine Transporter, in HEK293 Cells, a Human Blood-Brain Barrier Model Cell, and CCDSs Patient-Derived Fibroblasts
Sign Up to like & getrecommendations! Published in 2020 at "Pharmaceutical Research"
DOI: 10.1007/s11095-020-2779-0
Abstract: Cyclocreatine, a creatine analog, is a candidate drug for treating patients with cerebral creatine deficiency syndromes (CCDSs) caused by creatine transporter (CRT, SLC6A8) deficiency, which reduces brain creatine level. The purpose of this study was… read more here.
Keywords: creatine; ccdss patient; hek293 cells; cyclocreatine ... See more keywords
A cancer/testis antigen, NY-SAR-35, induces EpCAM, CD44, and CD133, and activates ERK in HEK293 cells.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.01.105
Abstract: The cancer/testis (CT) antigen NY-SAR-35 gene is located on the X chromosome and is aberrantly expressed in various cancers but not in normal tissues, other than testes. Previously, we reported the expression of NY-SAR-35 enhanced… read more here.
Keywords: hek293; hek293 cells; cancer testis; sar ... See more keywords
In vitro renal toxicity evaluation of copper-based metal-organic framework HKUST-1 on human embryonic kidney cells.
Sign Up to like & getrecommendations! Published in 2021 at "Environmental pollution"
DOI: 10.1016/j.envpol.2021.116528
Abstract: HKUST-1 is currently studied for a very diverse range of applications. Despite its exciting potential, significant concerns remain regarding the safety of HKUST-1. Therefore, human embryonic kidney 293 (HEK293) cells were used to verify the… read more here.
Keywords: embryonic kidney; renal toxicity; hek293 cells; hkust ... See more keywords
Apigenin attenuates patulin‐induced apoptosis in HEK293 cells by modulating ROS‐mediated mitochondrial dysfunction and caspase signal pathway
Sign Up to like & getrecommendations! Published in 2017 at "Toxicon"
DOI: 10.1016/j.toxicon.2017.07.018
Abstract: ABSTRACT Mycotoxins like patulin (PAT) are among the most significant food contaminant with regard to public health. This study aimed to evaluate the protective effect of apigenin (API), one of the most bioactive flavonoids in… read more here.
Keywords: patulin induced; ros mediated; apigenin; apoptosis hek293 ... See more keywords
Modulation of the cardiac sodium channel NaV1.5 peak and late currents by NAD+ precursors.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of molecular and cellular cardiology"
DOI: 10.1016/j.yjmcc.2020.01.013
Abstract: RATIONALE The cardiac sodium channel NaV1.5, encoded by SCN5A, produces the rapidly inactivating depolarizing current INa that is responsible for the initiation and propagation of the cardiac action potential. Acquired and inherited dysfunction of NaV1.5… read more here.
Keywords: cells expressing; ina; hek293 cells; cardiac sodium ... See more keywords