Investigation of the putative rate‐limiting role of electron transfer in fatty acid desaturation using transfected HEK293T cells
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13622
Abstract: Elevated fatty acid (FA) levels contribute to severe metabolic diseases. Unbalanced oversupply of saturated FAs is particularly damaging, which renders stearoyl‐CoA desaturase (SCD1) activity an important factor of resistance. A SCD1‐related oxidoreductase protects cells against… read more here.
Keywords: hek293t cells; fatty acid; electron transfer; rate limiting ... See more keywords
Knockout of Pro‐Apoptotic BAX and BAK1 Genes in HEK293T Cells Enhances Adeno‐Associated Virus (AAV) Production: AAV2 and AAV9
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Journal"
DOI: 10.1002/biot.202400529
Abstract: Increasing demand for adeno‐associated virus (AAV) used in gene therapy highlights the need to enhance AAV production. When intracellular AAV2 and extracellular AAV9 were produced in HEK293T cells using the triple transfection method, apoptosis occurred… read more here.
Keywords: hek293t cells; aav production; aav2 aav9; production ... See more keywords
Graphene oxide nanosheets induce DNA damage and activate the base excision repair (BER) signaling pathway both in vitro and in vivo.
Sign Up to like & getrecommendations! Published in 2017 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2017.06.049
Abstract: Graphene oxide (GO) has widespread concerns in the fields of biological sciences and medical applications. Currently, studies have reported that excessive GO exposure can cause cellular DNA damage through reactive oxygen species (ROS) generation. However,… read more here.
Keywords: graphene oxide; dna damage; hek293t cells; exposure ... See more keywords
CRISPR-Cas9-Mediated ATF6B Gene Editing Enhances Membrane Protein Production in HEK293T Cells
Sign Up to like & getrecommendations! Published in 2025 at "Bioengineering"
DOI: 10.3390/bioengineering12040409
Abstract: This study aims to enhance membrane protein production in HEK293T cells through genetic modification. HEK293T cells are used for recombinant protein and viral vector production due to their human origin and post-translational modification capabilities. This… read more here.
Keywords: protein; hek293t cells; protein production; production ... See more keywords
Large chromosomal deletions and impaired homologous recombination repairing in HEK293T cells exposed to polychlorinated biphenyl 153
Sign Up to like & getrecommendations! Published in 2021 at "PeerJ"
DOI: 10.7717/peerj.11816
Abstract: Background Polychlorinated biphenyls (PCBs) are persistent pollutants with carcinogenesis and mutagenesis effects which have been closely associated with PCBs-induced DNA damage. However, the detailed DNA damage events and corresponding pathway alterations under PCBs poisoning is… read more here.
Keywords: hek293t cells; large chromosomal; homologous recombination; chromosomal deletions ... See more keywords