Irc3 is a monomeric DNA branch point‐binding helicase in mitochondria of the yeast Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2020 at "FEBS Letters"
DOI: 10.1002/1873-3468.13893
Abstract: Irc3 is a superfamily II DNA helicase required for the maintenance of mitochondrial DNA stability in Saccharomyces cerevisiae. Here, we show that recombinant Irc3 is a monomeric protein and that it can form a binary… read more here.
Keywords: saccharomyces cerevisiae; dna; point; irc3 monomeric ... See more keywords
The apo‐form of the Vibrio cholerae replicative helicase DnaB is a labile and inactive planar trimer of dimers
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14403
Abstract: To enable chromosomal replication, DNA is unwound by the ATPase molecular motor replicative helicase. The bacterial helicase DnaB is a ring‐shaped homo‐hexamer whose conformational dynamics are being studied through its different 3D structural states adopted… read more here.
Keywords: trimer dimers; dnab; vibrio cholerae; replicative helicase ... See more keywords
How Does a Helicase Unwind DNA? Insights from RecBCD Helicase
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DOI: 10.1002/bies.201800009
Abstract: DNA helicases are a class of molecular motors that catalyze processive unwinding of double stranded DNA. In spite of much study, we know relatively little about the mechanisms by which these enzymes carry out the… read more here.
Keywords: unwind dna; recbcd helicase; dna; helicase unwind ... See more keywords
Challenges for the Discovery of Non‐Covalent WRN Helicase Inhibitors
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DOI: 10.1002/cmdc.202300613
Abstract: The Werner Syndrome RecQ helicase (WRN) is a synthetic lethal target of interest for the treatment of cancers with microsatellite instability (MSI). Different hit finding approaches were initially tested. The identification of WRN inhibitors proved… read more here.
Keywords: helicase inhibitors; helicase; discovery non; wrn helicase ... See more keywords
Repetitive Loop Formation by the CRISPR-Cas3 Helicase
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DOI: 10.1016/j.bpj.2016.11.430
Abstract: E. coli maintain CRISPR-Cas adaptive immune systems to protect the cell against invading genetic elements. Immunity relies on the RNA guided surveillance complex Cascade (CRISPR-associated complex for antiviral defense) and the trans-acting Cas3 protein with… read more here.
Keywords: loop formation; crispr; repetitive loop; cas3 ... See more keywords
Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2020.11.2276
Abstract: The superfamily 1 helicase nonstructural protein 13 (nsp13) is required for SARS-CoV-2 replication. The mechanism and regulation of nsp13 has not been explored at the single-molecule level. Specifically, force-dependent unwinding experiments have yet to be… read more here.
Keywords: dependent stimulation; rna; helicase; sars cov ... See more keywords
Inhibitory mechanism of 5-bromo-3-indoleacetic acid for non-structural-3 helicase hepatitis C virus with dynamics correlation network analysis
Sign Up to like & getrecommendations! Published in 2018 at "Computational biology and chemistry"
DOI: 10.1016/j.compbiolchem.2018.10.006
Abstract: Hepatitis C virus, Nonstructural 3 helicase (NS3h) protein is a well-studied segment of Non-structural 3/4 A helicase-protease protein that is crucial for the RNA duplex unwinding and RNA translocation during the process of HCV replication. Similar… read more here.
Keywords: inhibitory mechanism; non structural; rna binding; helicase ... See more keywords
Repetitive DNA Reeling by the Cascade-Cas3 Complex in Nucleotide Unwinding Steps.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.03.031
Abstract: CRISPR-Cas provides RNA-guided adaptive immunity against invading genetic elements. Interference in type I systems relies on the RNA-guided Cascade complex for target DNA recognition and the Cas3 helicase/nuclease protein for target degradation. Even though the… read more here.
Keywords: dna; repetitive dna; dna reeling; target ... See more keywords
CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation
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DOI: 10.1016/j.neuron.2019.09.003
Abstract: Hexanucleotide GGGGCC repeat expansion in C9ORF72 is the most prevalent genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). One pathogenic mechanism is the aberrant accumulation of dipeptide repeat (DPR) proteins produced by the unconventional… read more here.
Keywords: crispr cas9; repeat; translation; ggggcc ... See more keywords
Closing in on ATPase Activity by an RNA Helicase.
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DOI: 10.1016/j.str.2020.01.007
Abstract: In this issue of Structure, Absmeier et al. (2020) describe the molecular mechanisms employed by an RNA helicase to prevent premature ATP hydrolysis upon nucleotide binding. read more here.
Keywords: closing atpase; rna helicase; activity rna; helicase ... See more keywords
Structure-function analysis of DEAD-box helicase DDX43.
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DOI: 10.1016/j.ymeth.2022.03.002
Abstract: DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. It contains a K homology (KH) domain in its N terminus, a helicase core domain in… read more here.
Keywords: helicase; ddx43; domain; dead box ... See more keywords