Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202100702
Abstract: Type 1 secretion systems (T1SS) have a relatively simple architecture compared to other classes of secretion systems and therefore, are attractive to be optimized by protein engineering. Here, we report a KnowVolution campaign for the… read more here.
Keywords: region; hly enhancer; secretion; hemolysin ... See more keywords
Complex Multilevel Control of Hemolysin Production by Uropathogenic Escherichia coli
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DOI: 10.1128/mbio.02248-19
Abstract: Uropathogenic E. coli (UPEC) is the major cause of urinary tract infections and a frequent cause of sepsis. Nearly half of all UPEC strains produce the potent cytotoxin hemolysin, and its expression is associated with… read more here.
Keywords: hemolysin; coli; hemolysin production; activity ... See more keywords
Inhibition of Staphylococcus aureus α-Hemolysin Production Using Nanocurcumin Capped Au@ZnO Nanocomposite
Sign Up to like & getrecommendations! Published in 2022 at "Bioinorganic Chemistry and Applications"
DOI: 10.1155/2022/2663812
Abstract: Nanoparticles of gold with zinc oxide (Au@ZnO NPs) were prepared by laser ablation and then capped with curcumin nanoparticles (Cur-Au@ZnO NPs). The synthesized NPs were characterized using different techniques, including transmission electron microscopy (TEM), Fourier-transform… read more here.
Keywords: cur zno; spectroscopy; hemolysin; zno nps ... See more keywords
Differential expression of hemolysin genes in weakly and strongly hemolytic Brachyspira hyodysenteriae strains
Sign Up to like & getrecommendations! Published in 2020 at "BMC Veterinary Research"
DOI: 10.1186/s12917-020-02385-5
Abstract: Swine dysentery (SD) is a diarrheal disease in fattening pigs that is caused by the strongly hemolytic species Brachyspira (B.) hyodysenteriae, B. hampsonii and B. suanatina. As weakly hemolytic Brachyspira spp. are considered less virulent… read more here.
Keywords: hemolysin; hemolysin genes; time; hyodysenteriae ... See more keywords
Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Cell Factories"
DOI: 10.1186/s12934-019-1094-0
Abstract: BackgroundThe hemolysin (Hly) secretion system of E. coli allows the one-step translocation of hemolysin A (HlyA) from the bacterial cytoplasm to the extracellular medium, without a periplasmic intermediate. In this work, we investigate whether the… read more here.
Keywords: hemolysin; secretion system; secretion; system ... See more keywords
Dysregulated hemolysin liberates bacterial outer membrane vesicles for cytosolic lipopolysaccharide sensing
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DOI: 10.1371/journal.ppat.1007240
Abstract: Inflammatory caspase-11/4/5 recognize cytosolic LPS from invading Gram-negative bacteria and induce pyroptosis and cytokine release, forming rapid innate antibacterial defenses. Since extracellular or vacuole-constrained bacteria are thought to rarely access the cytoplasm, how their LPS… read more here.
Keywords: hemolysin; bacterial outer; membrane vesicles; outer membrane ... See more keywords
α-Hemolysin promotes uropathogenic E. coli persistence in bladder epithelial cells via abrogating bacteria-harboring lysosome acidification
Sign Up to like & getrecommendations! Published in 2023 at "PLOS Pathogens"
DOI: 10.1371/journal.ppat.1011388
Abstract: There is a growing consensus that a significant proportion of recurrent urinary tract infections are linked to the persistence of uropathogens within the urinary tract and their re-emergence upon the conclusion of antibiotic treatment. Studies… read more here.
Keywords: persistence; bladder; bacteria harboring; bladder epithelial ... See more keywords
GTP-Dependent FlhF Homodimer Supports Secretion of a Hemolysin in Bacillus cereus
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DOI: 10.3389/fmicb.2020.00879
Abstract: The multidomain (B-NG) protein FlhF, a flagellar biogenesis regulator in several bacteria, is the third paralog of the signal recognition particle (SRP)-GTPases Ffh and FtsY, which are known to drive protein-delivery to the plasma membrane.… read more here.
Keywords: hemolysin; secretion; bacillus cereus; gtp dependent ... See more keywords
2,3-Dehydrokievitone combats methicillin-resistant Staphylococcus aureus infection by reducing alpha-hemolysin expression
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.969215
Abstract: Due to powerful drug resistance and fatal toxicity of methicillin-resistant Staphylococcus aureus (MRSA), therapeutic strategies against virulence factors present obvious advantages since no evolutionary pressure will induce bacterial resistance. Alpha-hemolysin (Hla) is an extracellular toxin… read more here.
Keywords: methicillin resistant; expression; staphylococcus aureus; hemolysin ... See more keywords
ppGpp, the General Stress Response Alarmone, Is Required for the Expression of the α-Hemolysin Toxin in the Uropathogenic Escherichia coli Isolate, J96
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232012256
Abstract: ppGpp is an intracellular sensor that, in response to different types of stress, coordinates the rearrangement of the gene expression pattern of bacteria to promote adaptation and survival to new environmental conditions. First described to… read more here.
Keywords: escherichia coli; expression; hemolysin; isolate j96 ... See more keywords