Malaria Pigment Crystals: The Achilles′ Heel of the Malaria Parasite
Sign Up to like & getrecommendations! Published in 2021 at "Chemmedchem"
DOI: 10.1002/cmdc.202000895
Abstract: The biogenic formation of hemozoin crystals, a crucial process in heme detoxification by the malaria parasite, is reviewed as an antimalarial drug target. We first focus on the in‐vivo formation of hemozoin. A model is… read more here.
Keywords: malaria parasite; digestive vacuole; crystals achilles; hemozoin ... See more keywords
Hemozoin-mediated inflammasome activation limits long-lived anti-malarial immunity
Sign Up to like & getrecommendations! Published in 2021 at "Cell reports"
DOI: 10.1016/j.celrep.2021.109586
Abstract: SUMMARY During acute malaria, most individuals mount robust inflammatory responses that limit parasite burden. However, long-lived sterilizing anti-malarial memory responses are not efficiently induced, even following repeated Plasmodium exposures. Using multiple Plasmodium species, genetically modified… read more here.
Keywords: inflammasome activation; anti malarial; hemozoin; plasmodium ... See more keywords
Quantification of Free Ferriprotoporphyrin IX Heme and Hemozoin for Artemisinin Sensitive versus Delayed Clearance Phenotype Plasmodium falciparum Malarial Parasites.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00959
Abstract: We use Plasmodium falciparum culture synchronization, optimized heme and hemozoin extraction protocols, and mass spectrometry to quantify the abundance of free ferriprotoporphyrin IX (FPIX) heme and crystallized FPIX (hemozoin; Hz) for various growth stages of… read more here.
Keywords: falciparum malarial; malarial parasites; heme hemozoin; hemozoin ... See more keywords
Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09122-z
Abstract: Methods to diagnose malaria are of paramount interest to eradicate the disease. Current methods have severe limitations, as they are either costly or not sensitive enough to detect low levels of parasitemia. Here we report… read more here.
Keywords: catalyzed precipitation; polymerization; hemozoin; precipitation polymerization ... See more keywords
Portable Device for Optical Quantification of Hemozoin in Diluted Blood Samples
Sign Up to like & getrecommendations! Published in 2020 at "IEEE Transactions on Biomedical Engineering"
DOI: 10.1109/tbme.2019.2913454
Abstract: Objective: This paper focuses on a novel and portable device prototype with optical detectors to quickly and efficiently detect hemozoin (Hz) in blood, aiming at malaria diagnostics. Methods: Taking advantage of the particular features of… read more here.
Keywords: hemozoin; blood; portable device; device optical ... See more keywords
Plasmodium berghei Purified Hemozoin Associated with DNA Strongly Inhibits P. berghei Liver-Stage Development in BALB/c Mice after Intravenous Inoculation
Sign Up to like & getrecommendations! Published in 2023 at "Infection and Immunity"
DOI: 10.1128/iai.00304-22
Abstract: In the acidic lysosome-like digestive vacuole, Plasmodium parasites crystallize heme from hemoglobin into hemozoin, or malaria pigment. Upon release of progeny merozoites, the residual hemozoin is phagocytized by macrophages principally in the liver and spleen… read more here.
Keywords: plasmodium; plasmodium berghei; hemozoin; liver stage ... See more keywords
Identification of proteins for controlled nucleation of metal-organic crystals for nanoenergetics
Sign Up to like & getrecommendations! Published in 2019 at "MRS Communications"
DOI: 10.1557/mrc.2019.46
Abstract: Here, we report that a marine sandworm Nereis virens jaw protein, Nvjp1, nucleates hemozoin with similar activity as the native parasite hemozoin protein, HisRPII. X-ray diffraction and scanning electron microscopy confirm the identity of the… read more here.
Keywords: hemozoin; nucleation metal; controlled nucleation; metal organic ... See more keywords
Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecules"
DOI: 10.3390/biom9100579
Abstract: The rotating-crystal magneto-optical diagnostic (RMOD) technique was developed as a sensitive and rapid platform for malaria diagnosis. Herein, we report a detailed in vivo assessment of the synchronized Plasmodium vinckei lentum strain blood-stage infections by… read more here.
Keywords: malaria; hemozoin; blood; blood stage ... See more keywords
Field Evaluation of a Hemozoin-Based Malaria Diagnostic Device in Puerto Lempira, Honduras
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostics"
DOI: 10.3390/diagnostics12051206
Abstract: The diagnosis of malaria in Honduras is based mainly on microscopic observation of the parasite in thick smears or the detection of parasite antigens through rapid diagnostic tests when microscopy is not available. The specific… read more here.
Keywords: sensitivity; gazelle; hemozoin; microscopy ... See more keywords