A modified multiparametric assay using HepaRG cells for predicting the degree of drug‐induced liver injury risk
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Applied Toxicology"
DOI: 10.1002/jat.3371
Abstract: The approach for predicting the degree of drug‐induced liver injury (DILI) risk was investigated quantitatively in a modified multiparametric assay using HepaRG cells. Thirty‐eight drugs were classified by DILI risk into five categories based on… read more here.
Keywords: risk; modified multiparametric; heparg cells; drug ... See more keywords
Setup and Use of HepaRG Cells in Cholestasis Research.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_19
Abstract: Since HepaRG cells can differentiate into well-polarized mature hepatocyte-like cells that synthesize, conjugate, and secrete bile acids, they represent an appropriate surrogate to primary human hepatocytes for investigations on drug-induced cholestasis mechanisms. In this chapter,… read more here.
Keywords: cells cholestasis; bile acids; setup use; heparg cells ... See more keywords
ZIP14 is degraded in response to manganese exposure
Sign Up to like & getrecommendations! Published in 2019 at "BioMetals"
DOI: 10.1007/s10534-019-00216-1
Abstract: Manganese (Mn) is an essential element necessary for proper development and brain function. Circulating Mn levels are regulated by hepatobiliary clearance to limit toxic levels and prevent tissue deposition. To characterize mechanisms involved in hepatocyte… read more here.
Keywords: degraded response; heparg cells; exposure; zip14 degraded ... See more keywords
Evaluation of HepaRG cells for the assessment of indirect drug-induced hepatotoxicity using INH as a model substance
Sign Up to like & getrecommendations! Published in 2017 at "Human Cell"
DOI: 10.1007/s13577-017-0175-9
Abstract: HepaRG cells are widely used as an in vitro model to assess drug-induced hepatotoxicity. However, only few studies exist so far regarding their suitability to detect the effects of drugs requiring a preceding activation via… read more here.
Keywords: substance; heparg cells; heparg; drug induced ... See more keywords
3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model.
Sign Up to like & getrecommendations! Published in 2020 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2020.120611
Abstract: Bioprinting is an emergent technology that has already demonstrated the capacity to create complex and/or vascularized multicellular structures with defined and organized architectures, in a reproducible and high throughput way. Here, we present the implementation… read more here.
Keywords: culture heparg; heparg cells; cells gelma; heparg ... See more keywords
Metabolic Signature of Ethanol-Induced Hepatotoxicity in HepaRG Cells by Liquid Chromatography-Mass Spectrometry-Based Untargeted Metabolomics.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00029
Abstract: Alcoholic liver disease is highly prevalent but poorly identified and characterized, leading to knowledge gaps, which impairs early diagnosis. Excessive alcohol consumption is known to alter lipid metabolism, followed by progressive intracellular lipid accumulation, resulting… read more here.
Keywords: liquid chromatography; ethanol induced; mass spectrometry; induced hepatotoxicity ... See more keywords
In Vitro Identification of Potential Metabolites of Plinabulin (NPI 2358) in Hepatic Preparations Using Liquid Chromatography–Ion Trap Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c00929
Abstract: Plinabulin (1, NPI2358), a vascular disrupting agent (VDA) molecule, is a synthetic analogue of the natural product phenylahistin (2, NPI 2350), which is isolated from Aspergillus ustus. Evaluation of the in vitro metabolic profile of… read more here.
Keywords: chromatography ion; cells cryopreserved; liquid chromatography; hlms heparg ... See more keywords
Functional polarization of human hepatoma HepaRG cells in response to forskolin
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-34421-8
Abstract: HepaRG is an original human hepatoma cell line, acquiring highly differentiated hepatic features when exposed to dimethylsulfoxide (DMSO). To search alternatives to DMSO, which may exert some toxicity, we have analyzed the effects of forskolin… read more here.
Keywords: human hepatoma; functional polarization; heparg cells; heparg ... See more keywords
From the Cover: MechanisticInsights in Cytotoxic and Cholestatic Potential of the Endothelial Receptor Antagonists Using HepaRG Cells
Sign Up to like & getrecommendations! Published in 2017 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfx062
Abstract: Several endothelin receptor antagonists (ERAs) have been developed for the treatment of pulmonary arterial hypertension (PAH). Some of them have been related to clinical cases of hepatocellular injury (sitaxentan [SIT]) and/or cholestasis (bosentan [BOS]). We… read more here.
Keywords: mechanisticinsights cytotoxic; heparg cells; cover mechanisticinsights; receptor antagonists ... See more keywords
A Dynamic Mathematical Model of Bile Acid Clearance in HepaRG Cells
Sign Up to like & getrecommendations! Published in 2018 at "Toxicological Sciences"
DOI: 10.1093/toxsci/kfx199
Abstract: A dynamic model based on ordinary differential equations that describes uptake, basolateral and canalicular export of taurocholic acid (TCA) in human HepaRG cells is presented. The highly reproducible inter-assay experimental data were used to reliably… read more here.
Keywords: heparg cells; tca clearance; model; clearance ... See more keywords
HepaRG Cells Adopt Zonal-Like Drug-Metabolizing Phenotypes under Physiologically Relevant Oxygen Tensions and Wnt/β-Catenin Signaling
Sign Up to like & getrecommendations! Published in 2022 at "Drug Metabolism and Disposition"
DOI: 10.1124/dmd.122.000870
Abstract: The cellular microenvironment plays an important role in liver zonation, the spatial distribution of metabolic tasks among hepatocytes lining the sinusoid. Standard tissue culture practices provide an excess of oxygen and a lack of signaling… read more here.
Keywords: oxygen tensions; wnt catenin; oxygen; catenin signaling ... See more keywords