Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans
Sign Up to like & getrecommendations! Published in 2018 at "Annals of Microbiology"
DOI: 10.1007/s13213-018-1347-x
Abstract: Heparinases are widely used for production of clinically and therapeutically important bioactive oligosaccharides and in analyzing the polydisperse, heterogeneous, and complex structures of heparin/heparan sulfate. In the present study, the gene (1911 bp) encoding heparinase II/III… read more here.
Keywords: polysaccharide lyase; heparinase iii; pspl12a; pseudopedobacter saltans ... See more keywords
82 Heparinase-Native Thromboelastometry Detects Hypercoagulability in COVID-19 Disease
Sign Up to like & getrecommendations! Published in 2021 at "Annals of Emergency Medicine"
DOI: 10.1016/j.annemergmed.2021.07.084
Abstract: Study Objectives: COVID-19 disease is associated with elevated risk of thrombosis, but lab assessment of hypercoagulability of fibrinolysis using conventional clotting assays is challenging. Rotational thromboelastometry (ROTEM) can detect subtle changes in clotting activity and… read more here.
Keywords: disease; rotem; hypercoagulability; naheptem ... See more keywords
Uncovering the detailed mode of cleavage of heparinase I toward structurally defined heparin oligosaccharides.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.08.260
Abstract: For a more insightful investigation into the specificity of bacterial heparinase I, a series of structurally well-defined heparin oligosaccharides was synthesized using a highly efficient chemoenzymatic strategy. Apart from the primary cleavage site, five glycosidic… read more here.
Keywords: uncovering detailed; cleavage; defined heparin; heparin oligosaccharides ... See more keywords
Optimizing miR-29 measurements in biobanked, heparinized samples.
Sign Up to like & getrecommendations! Published in 2019 at "Life sciences"
DOI: 10.1016/j.lfs.2019.116894
Abstract: AIMS MicroRNAs (miRs) and their importance in development, normal physiology, and disease have become increasingly recognized. Our laboratory is interested in miR-29 and its effects on lung development. These studies set out to identify optimal… read more here.
Keywords: mir; heparinus heparinase; recombinant heparinus; optimizing mir ... See more keywords
Immobilization alters heparin cleaving properties of heparinase I
Sign Up to like & getrecommendations! Published in 2017 at "Glycobiology"
DOI: 10.1093/glycob/cwx074
Abstract: We report here a novel observation that immobilization of heparinase I on CNBr-activated Sepharose results in heparin degradation properties that are different from heparinase I in the free solution form. Studies over a range of… read more here.
Keywords: immobilization; heparinase immobilization; heparin cleaving; immobilization alters ... See more keywords
Isolation and characterization of HepP: a virulence-related Pseudomonas aeruginosa heparinase
Sign Up to like & getrecommendations! Published in 2017 at "BMC Microbiology"
DOI: 10.1186/s12866-017-1141-0
Abstract: BackgroundPseudomonas aeruginosa is an opportunistic pathogen that causes serious infections in immunocompromised hosts including severely burned patients. In burn patients, P. aeruginosa infection often leads to septic shock and death. Despite numerous studies, the influence… read more here.
Keywords: virulence; burned patients; hepp; pa14 ... See more keywords
Cloning, Expression, and Characterization of a Highly Stable Heparinase I from Bacteroides xylanisolvens
Sign Up to like & getrecommendations! Published in 2023 at "Polymers"
DOI: 10.3390/polym15071776
Abstract: Heparinase I (Hep I), which specifically degrades heparin to oligosaccharide or unsaturated disaccharide, has an important role in the production of low molecular weight heparin (LMWH). However, low productivity and stability of heparinase I hinders… read more here.
Keywords: heparinase; bacteroides xylanisolvens; activity; expression characterization ... See more keywords