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Heteroduplex cleavage assay for screening of probable zygosities resulting from CRISPR mutations in diploid single cell lines.

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1 Published in 2017 at "BioTechniques"

DOI: 10.2144/000114556

Abstract: The most common gene editing methods, such as CRISPR, involve random repair of an induced double-stranded DNA break through the non-homologous end joining (NHEJ) repair pathway, resulting in small insertions/deletions. In diploid cells, these mutations… read more here.

Keywords: gene editing; dna sequencing; heteroduplex cleavage; gene ... See more keywords