Heterologous Expression of an Unusual Ketosynthase, SxtA, Leads to Production of Saxitoxin Intermediates in Escherichia coli
Sign Up to like & getrecommendations! Published in 2020 at "ChemBioChem"
DOI: 10.1002/cbic.202000675
Abstract: Paralytic shellfish toxins (PSTs) are neurotoxic alkaloids produced by freshwater cyanobacteria and marine dinoflagellates. Due to their antagonism of voltage‐gated sodium channels in excitable cells, certain analogues are of significant pharmacological interest. The biosynthesis of… read more here.
Keywords: unusual ketosynthase; sxta; escherichia coli; expression unusual ... See more keywords
Enhancing the biosynthesis of nicotinamide mononucleotide in Lactococcus lactis by heterologous expression of FtnadE.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the science of food and agriculture"
DOI: 10.1002/jsfa.12253
Abstract: BACKGROUND Nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide, plays an important in anti-aging and disease. Lactococcus lactis, an important probiotic lactic acid bacteria (LAB), has shown great potential for the biosynthesis of… read more here.
Keywords: nicotinamide mononucleotide; biosynthesis; heterologous expression; lactis ... See more keywords
Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity Via Heterologous Expression in Fission Yeast.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2245-2_6
Abstract: Heterologous expression of cyclic nucleotide phosphodiesterases (PDEs) and adenylyl cyclases (ACs) in the fission yeast Schizosaccharomyces pombe can be used in combination with PKA-repressed reporters to either carry out high throughput screens for small molecule… read more here.
Keywords: heterologous expression; fission yeast; activity;
Heterologous Expression and Patch-Clamp Recording of P2X Receptors in HEK293 Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9717-6_19
Abstract: P2X receptors (P2XRs) are ligand-gated ion channels gated by extracellular adenosine 5'-triphosphate (ATP) and play a critical role in mediating ATP-induced purinergic signaling in physiological and pathological processes. Heterologous expression of P2XR in human embryonic… read more here.
Keywords: p2x receptors; patch clamp; hek293 cells; clamp recording ... See more keywords
Heterologous expression of wheat TaRUB1 gene enhances disease resistance in Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2017 at "Plant Cell Reports"
DOI: 10.1007/s00299-017-2221-1
Abstract: Key messageExpression ofTaRUB1gene inArabidopsis thalianaelevates the level of disease-related genes in response to pathogen invasion through the accumulation of callose, necrotic cells, and the outbreak of ROS.AbstractUbiquitin (Ub) and ubiquitin-like proteins are highly conserved in… read more here.
Keywords: wheat; disease resistance; thaliana; resistance ... See more keywords
Effect of Heterologous Expression of Chemotaxis Proteins from Genus Thermotoga on the Growth Kinetics of Escherichia coli Cells
Sign Up to like & getrecommendations! Published in 2019 at "Bulletin of Experimental Biology and Medicine"
DOI: 10.1007/s10517-019-04530-z
Abstract: In the process of optimization of heterologous expression of thermostable chemotaxis proteins CheW and CheY as industrially useful polypeptides, their direct influence on the cell growth kinetics and morphology of Escherichia coli was observed. CheW… read more here.
Keywords: growth kinetics; escherichia coli; chemotaxis proteins; heterologous expression ... See more keywords
Heterologous expression, purification, and refolding of SRY protein: role of l-arginine as analyzed by simulation and practical study
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biology Reports"
DOI: 10.1007/s11033-020-05667-1
Abstract: Escherichia coli is a widely-used cell factory for recombinant protein production, nevertheless, high amount of produced protein is seen in aggregated form. The purpose of this study was to improve the solubility of recombinant bovine… read more here.
Keywords: purification refolding; study; arginine; refolding sry ... See more keywords
Transcriptional heterologous expression of two type III PKS from the lichen Cladonia uncialis
Sign Up to like & getrecommendations! Published in 2019 at "Mycological Progress"
DOI: 10.1007/s11557-019-01539-6
Abstract: Type III polyketide synthases (PKS) are an under-explored group of enzymes that are responsible for producing a variety of bioactive molecules. In a previous study, we identified two type III PKS genes (t3pks1 and t3pks2)… read more here.
Keywords: heterologous expression; iii pks; type iii; pks ... See more keywords
Autoinduction as Means for Optimization of the Heterologous Expression of Recombinant Single-Chain Fv (scFv) Antibodies
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Biotechnology"
DOI: 10.1007/s12033-021-00363-2
Abstract: The monoclonal antibodies and the recombinant antibody fragments are widely used in the biotechnology studies and in medicine as a powerful therapeutic and diagnostic tool. The most commonly used recombinant antibody fragments are single-chain fragment… read more here.
Keywords: heterologous expression; single chain; expression; scfv antibodies ... See more keywords
Enhancement of welan gum production in Sphingomonas sp. HT-1 via heterologous expression of Vitreoscilla hemoglobin gene.
Sign Up to like & getrecommendations! Published in 2017 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2016.08.081
Abstract: Welan gum is a microbial polysaccharide produced by Sphingomonas sp. Its production is limited by the dissolved oxygen levels in the highly viscous fermentation. A strategy of heterologous expression of the Vitreoscilla hemoglobin gene in… read more here.
Keywords: expression vitreoscilla; production; welan gum; welan ... See more keywords
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2020.12.016
Abstract: The heterologous expression of natural product biosynthetic gene clusters (BGCs) has traditionally been used as a genetic platform to link various natural product chemotypes to their corresponding genotypes. In recent years, heterologous expression has played… read more here.
Keywords: expression natural; bgcs; natural product; heterologous expression ... See more keywords