Application of hydrocarbon and perfluorocarbon oxygen vectors to enhance heterologous production of hyaluronic acid in engineered Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26551
Abstract: In microbial cultivations for hyaluronic acid (HA) production, oxygen can be a limiting substrate due to its poor solubility in aqueous medium and the substantial increase in culture viscosity at relatively low HA titers. Shear… read more here.
Keywords: oxygen; oxygen vectors; production; heterologous production ... See more keywords
Coexpression of 5‐Aminolevulinic Acid Synthase Gene Facilitates Heterologous Production of Thermostable Cytochrome P450, CYP119, in Holo Form in Escherichia coli
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800331
Abstract: Cytochrome P450 enzymes are heme‐containing monooxygenases that exhibit potential as biocatalysts for practical applications. The Escherichia coli expression system is frequently used for biocatalyst production; however, heterologous production of hemeproteins in their holo form is… read more here.
Keywords: coexpression; holo form; production; heterologous production ... See more keywords
Crucial Role of the Chaperonin GroES/EL for Heterologous Production of the Soluble Methane Monooxygenase from Methylomonas methanica MC09
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200195
Abstract: Methane is a widespread energy source and can serve as an attractive C1 building block for a future bioeconomy. The soluble methane monooxygenase (sMMO) is able to break the strong C−H bond of methane and… read more here.
Keywords: heterologous production; production; methane monooxygenase; methane ... See more keywords
Violaxanthin: natural function and occurrence, biosynthesis, and heterologous production.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11452-2
Abstract: Violaxanthin is biosynthesized from zeaxanthin with zeaxanthin epoxidase (ZEP) by way of antheraxanthin only in photosynthetic eukaryotes including higher plants and involved in the xanthophyll cycle to eliminate excessive light energy. Violaxanthin and antheraxanthin have… read more here.
Keywords: function occurrence; production; heterologous production; natural function ... See more keywords
Heterologous production of resveratrol in bacterial hosts: current status and perspectives
Sign Up to like & getrecommendations! Published in 2018 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-018-2506-8
Abstract: The polyphenol resveratrol (3,5,4′-trihydroxystilbene) is a well-known plant secondary metabolite, commonly used as a medical ingredient and a nutritional supplement. Due to its health-promoting properties, the demand for resveratrol is expected to continue growing. This… read more here.
Keywords: resveratrol; production; heterologous production; bacterial hosts ... See more keywords
Biosynthetic Characterization, Heterologous Production, and Genomics-Guided Discovery of GABA-Containing Fungal Heptapeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of natural products"
DOI: 10.1021/acs.jnatprod.2c01065
Abstract: The biosynthetic gene cluster of γ-aminobutyric acid (GABA)-containing fungal cyclic heptapeptides unguisins A (1) and B (2) was identified in the fungus Aspergillus violaceofuscus CBS 115571. In vitro enzymatic reactions and gene deletion experiments revealed… read more here.
Keywords: containing fungal; heterologous production; biosynthetic characterization; gaba containing ... See more keywords
Heterologous Production of the C33-C45 Polyketide Fragment of Anticancer Apratoxins in a Cyanobacterial Host.
Sign Up to like & getrecommendations! Published in 2023 at "Organic letters"
DOI: 10.1021/acs.orglett.3c00462
Abstract: A polyketide synthase subcluster of cytotoxic apratoxin A was isolated from a Moorena bouillonii environmental DNA library and engineered with a thioesterase II domain for heterologous expression in the filamentous cyanobacterium Anabaena sp. PCC7120. Further… read more here.
Keywords: c33 c45; c45 polyketide; polyketide fragment; heterologous production ... See more keywords
Directing the Heterologous Production of Specific Cyanobacterial Toxin Variants.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00181
Abstract: Microcystins are globally the most commonly occurring freshwater cyanotoxins, causing acute poisoning and chronically inducing hepatocellular carcinoma. However, the detection and toxicological study of microcystins is hampered by the limited availability and high cost of… read more here.
Keywords: heterologous production; directing heterologous; microcystin; toxin ... See more keywords
Heterologous Production and Purification of a Functional Chloroform Reductive Dehalogenase.
Sign Up to like & getrecommendations! Published in 2018 at "ACS chemical biology"
DOI: 10.1021/acschembio.7b00846
Abstract: Reductive dehalogenases (RDases) are key enzymes involved in the respiratory process of anaerobic organohalide respiring bacteria (ORB). Heterologous expression of respiratory RDases is desirable for structural and functional studies; however, there are few reports of… read more here.
Keywords: heterologous production; reductive dehalogenase; expression; chloroform ... See more keywords
Heterologous production of levopimaric acid in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2018 at "Microbial Cell Factories"
DOI: 10.1186/s12934-018-0964-1
Abstract: BackgroundLevopimaric acid (LA), a type of diterpene resin acid produced by plants, is a significant industrial intermediate that is mainly produced via phytoextraction. This work aimed to apply synthetic biology to produce LA in yeast… read more here.
Keywords: production; production levopimaric; heterologous production; yeast ... See more keywords
Heterologous production of active form of beta-lytic protease by Bacillus subtilis and improvement of staphylolytic activity by protein engineering
Sign Up to like & getrecommendations! Published in 2021 at "Microbial Cell Factories"
DOI: 10.1186/s12934-021-01724-x
Abstract: Background Most of the proteases classified into the M23 family in the MEROPS database exhibit staphylolytic activity and have potential as antibacterial agents. The M23 family is further classified into two subfamilies, M23A and M23B.… read more here.
Keywords: blp; active form; production; staphylolytic activity ... See more keywords