YBac: A novel and simple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202200558
Abstract: The baculovirus expression vector system (BEVS) has been widely used for heterologous protein expression due to its powerful functionality and easy access to commercial expression vectors. Currently, most laboratories prefer two strategies for protein production… read more here.
Keywords: system; protein production; production; heterologous protein ... See more keywords
Selection of Heterologous Protein-Producing Strains in Yarrowia lipolytica.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_6
Abstract: Yarrowia lipolytica has emerged as an alternative expression system for heterologous protein production and enzyme evolution. Several different expression systems dedicated for this species have been developed, ranging from the simple cloning of expression vectors… read more here.
Keywords: producing strains; selection; expression; heterologous protein ... See more keywords
Fusion tags to enhance heterologous protein expression
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10402-8
Abstract: Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of proteins, insufficient yield, and inclusion body formation. Numerous approaches have sought to… read more here.
Keywords: protein expression; fusion tags; expression; heterologous protein ... See more keywords
Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production
Sign Up to like & getrecommendations! Published in 2021 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-021-02633-4
Abstract: Developing a Komagataella phaffii (K. phaffii, named as Pichia pastoris formerly) medium using wheat bran hydrolysate (WBH) is a potential approach for wheat bran utilization and heterologous protein by K. phaffii because K. phaffii is… read more here.
Keywords: medium; heterologous protein; phaffii; wheat bran ... See more keywords
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia pastoris yeast
Sign Up to like & getrecommendations! Published in 2018 at "Brazilian Journal of Microbiology"
DOI: 10.1016/j.bjm.2018.03.010
Abstract: Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use… read more here.
Keywords: protein; production; carbon; protein production ... See more keywords
Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria.
Sign Up to like & getrecommendations! Published in 2021 at "ACS infectious diseases"
DOI: 10.1021/acsinfecdis.1c00154
Abstract: Environmental and intracellular stresses can perturb protein homeostasis and trigger the formation and accumulation of protein aggregates. It has been recently suggested that the level of protein aggregates accumulated in bacteria correlates with the frequency… read more here.
Keywords: protein; viable nonculturable; protein aggregates; expression ... See more keywords
Phycocyanin Fusion Constructs for Heterologous Protein Expression Accumulate as Functional Heterohexameric Complexes in Cyanobacteria.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00449
Abstract: Overexpression of heterologous proteins from plants, bacteria, and human as fusion constructs in cyanobacteria has been documented in the literature. Typically, the heterologous protein "P" of interest is expressed as a fusion with the abundant… read more here.
Keywords: fusion; synthase; cpcb fusion; fusion constructs ... See more keywords
Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
Sign Up to like & getrecommendations! Published in 2017 at "Microbial Cell Factories"
DOI: 10.1186/s12934-016-0616-2
Abstract: BackgroundThe implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. Lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior… read more here.
Keywords: lactis; lactis nz9000; protein; lactococcus lactis ... See more keywords
Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
Sign Up to like & getrecommendations! Published in 2018 at "Microbial Cell Factories"
DOI: 10.1186/s12934-018-1040-6
Abstract: BackgroundThe Gram-positive Streptomyces lividans TK24 is an attractive host for heterologous protein production because of its high capability to secrete proteins—which favors correct folding and facilitates downstream processing—as well as its acceptance of methylated DNA… read more here.
Keywords: protein; protein production; streptomyces lividans; expression ... See more keywords
The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21020576
Abstract: This work assesses the effect of chemical induction with isopropyl β-D-1-thiogalactopyranoside (IPTG) on the expression of enhanced green fluorescent protein (eGFP) by planktonic and biofilm cells of Escherichia coli JM109(DE3) transformed with a plasmid containing… read more here.
Keywords: protein; escherichia coli; induction; expression ... See more keywords