Synthetic propeptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae
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DOI: 10.1002/mbo3.1300
Abstract: Heterologous protein production in Saccharomyces cerevisiae is a useful and effective strategy with many advantages, including the secretion of proteins that require posttranslational processing. However, heterologous proteins in S. cerevisiae are often secreted at comparatively… read more here.
Keywords: csf; production; secretion; design ... See more keywords
Display of Heterologous Proteins in Bacillus Subtilis Biofilms for Enteric Immunization.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2168-4_4
Abstract: One of the foremost goals in vaccine development is the design of effective, heat-stable vaccines that simplify the distribution and delivery while conferring high levels of protective immunity. Here, we describe a method for developing… read more here.
Keywords: proteins bacillus; heterologous proteins; subtilis biofilms; bacillus ... See more keywords
Production of extracellular heterologous proteins in Streptomyces rimosus, producer of the antibiotic oxytetracycline
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-8793-z
Abstract: Among the Streptomyces species, Streptomyces lividans has often been used for the production of heterologous proteins as it can secrete target proteins directly into the culture medium. Streptomyces rimosus, on the other hand, has for… read more here.
Keywords: oxytetracycline production; production; heterologous proteins; host ... See more keywords
Secretome-based screening of fusion partners and their application in recombinant protein secretion in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11750-9
Abstract: For the efficient production of heterologous proteins in the yeast Saccharomyces cerevisiae, we screened for a novel fusion partner from the yeast secretome. From twenty major proteins identified from the yeast secretome, we selected Scw4p,… read more here.
Keywords: heterologous proteins; fusion partner; fusion; secretion ... See more keywords
Systematic Screening of Optimal Signal Peptides for Secretory Production of Heterologous Proteins in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b04183
Abstract: Bacillus subtilis is widely used for large-scale industrial production of heterologous proteins. Because of its high intrinsic secretory capacity, it can efficiently secrete proteins into the culture supernatant via the general Sec-type secretion pathway. In… read more here.
Keywords: signal peptides; bacillus subtilis; production heterologous; heterologous proteins ... See more keywords
On display: autotransporter secretion and application.
Sign Up to like & getrecommendations! Published in 2018 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fny165
Abstract: The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins that pass the inner and… read more here.
Keywords: autotransporter secretion; secretion; heterologous proteins; outer membrane ... See more keywords
Identification of genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous proteins
Sign Up to like & getrecommendations! Published in 2022 at "PLOS Biology"
DOI: 10.1371/journal.pbio.3001877
Abstract: The yeast Komagataella phaffii (formerly called Pichia pastoris) is used widely as a host for secretion of heterologous proteins, but only a few isolates of this species exist and all the commonly used expression systems… read more here.
Keywords: phaffii; komagataella phaffii; pichia pastoris; yeast komagataella ... See more keywords