Long‐read HiFi sequencing of NUDT15: Phased full‐gene haplotyping and pharmacogenomic allele discovery
Sign Up to like & getrecommendations! Published in 2022 at "Human Mutation"
DOI: 10.1002/humu.24457
Abstract: To determine the phase of NUDT15 sequence variants for more comprehensive star (*) allele diplotyping, we developed a novel long‐read single‐molecule real‐time HiFi amplicon sequencing method. A 10.5 kb NUDT15 amplicon assay was validated using… read more here.
Keywords: read hifi; hifi; full gene; long read ... See more keywords
Analyzing rare mutations in metagenomes assembled using long and accurate reads
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/gr.276917.122
Abstract: The advent of long and accurate “HiFi” reads has greatly improved our ability to generate complete metagenome-assembled genomes (MAGs), enabling “complete metagenomics” studies that were nearly impossible to conduct with short reads. In particular, HiFi… read more here.
Keywords: assembled using; rare mutations; hifi; mutations mags ... See more keywords
Improved Assembly of Metagenome-Assembled Genomes and Viruses in Tibetan Saline Lake Sediment by HiFi Metagenomic Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.03328-22
Abstract: To expand the understanding of microbial dark matter in the environment, we did the first comparative evaluation of multiple assembly strategies based on high-throughput short-read and HiFi data from lake sediments metagenomic sequencing. The results… read more here.
Keywords: short read; hifi; assembly; hifi data ... See more keywords
Abstract 2934: Somatic variant workflow with HiFi sequencing provides new insights in highly challenging cancer cases
Sign Up to like & getrecommendations! Published in 2024 at "Cancer Research"
DOI: 10.1158/1538-7445.am2024-2934
Abstract: Tumor evolution is a highly heterogeneous process where multiple oncogenic pathways can lead to host defense evasion. Advancements in genome sequencing are allowing us to better understand these processes and their impact. However, critical genomic… read more here.
Keywords: cancer; structural variants; somatic variant; hifi sequencing ... See more keywords
Implementing Hifi Sequencing for Enhanced Genomic and Methylome Characterization in Multiple Myeloma
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DOI: 10.1182/blood-2024-207422
Abstract: Introduction: Over recent years, the introduction and scalability of next-generation sequencing technologies have significantly expanded our knowledge of the genetic underpinnings of multiple myeloma (MM), a heterogeneous hematologic malignancy characterized by clonal plasma cell proliferation… read more here.
Keywords: multiple myeloma; short read; analysis; coverage ... See more keywords
Nanoclay-Reinforced Glass-Ionomer Cements: In Vitro Wear Evaluation and Comparison by Two Wear-Test Methods
Sign Up to like & getrecommendations! Published in 2017 at "Dentistry Journal"
DOI: 10.3390/dj5040028
Abstract: Glass ionomer cement (GIC) represents a major transformation in restorative dentistry. Wear of dental restoratives is a common phenomenon and the determination of the wear resistance of direct-restorative materials is a challenging task. The aim… read more here.
Keywords: glass ionomer; wear test; volume; glass ... See more keywords