Long‐read HiFi sequencing of NUDT15: Phased full‐gene haplotyping and pharmacogenomic allele discovery
Sign Up to like & getrecommendations! Published in 2022 at "Human Mutation"
DOI: 10.1002/humu.24457
Abstract: To determine the phase of NUDT15 sequence variants for more comprehensive star (*) allele diplotyping, we developed a novel long‐read single‐molecule real‐time HiFi amplicon sequencing method. A 10.5 kb NUDT15 amplicon assay was validated using… read more here.
Keywords: read hifi; hifi; full gene; long read ... See more keywords
Analyzing rare mutations in metagenomes assembled using long and accurate reads
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DOI: 10.1101/gr.276917.122
Abstract: The advent of long and accurate “HiFi” reads has greatly improved our ability to generate complete metagenome-assembled genomes (MAGs), enabling “complete metagenomics” studies that were nearly impossible to conduct with short reads. In particular, HiFi… read more here.
Keywords: assembled using; rare mutations; hifi; mutations mags ... See more keywords
Improved Assembly of Metagenome-Assembled Genomes and Viruses in Tibetan Saline Lake Sediment by HiFi Metagenomic Sequencing
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DOI: 10.1128/spectrum.03328-22
Abstract: To expand the understanding of microbial dark matter in the environment, we did the first comparative evaluation of multiple assembly strategies based on high-throughput short-read and HiFi data from lake sediments metagenomic sequencing. The results… read more here.
Keywords: short read; hifi; assembly; hifi data ... See more keywords
Nanoclay-Reinforced Glass-Ionomer Cements: In Vitro Wear Evaluation and Comparison by Two Wear-Test Methods
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DOI: 10.3390/dj5040028
Abstract: Glass ionomer cement (GIC) represents a major transformation in restorative dentistry. Wear of dental restoratives is a common phenomenon and the determination of the wear resistance of direct-restorative materials is a challenging task. The aim… read more here.
Keywords: glass ionomer; wear test; volume; glass ... See more keywords