Full-length sequencing of CYP2D6 variants with PacBio HiFi reads
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Genetics and Metabolism"
DOI: 10.1016/s1096-7192(21)00507-2
Abstract: A two-step PCR with barcoded M13 Primers was used to enable pooling of 22 samples for a single SMRTbell library preparation, which was sequenced on the PacBio Sequel II and IIe Systems. HiFi reads (>QV20)… read more here.
Keywords: cyp2d6 variants; sequencing cyp2d6; length sequencing; full length ... See more keywords
Analyzing rare mutations in metagenomes assembled using long and accurate reads
Sign Up to like & getrecommendations! Published in 2022 at "Genome Research"
DOI: 10.1101/gr.276917.122
Abstract: The advent of long and accurate “HiFi” reads has greatly improved our ability to generate complete metagenome-assembled genomes (MAGs), enabling “complete metagenomics” studies that were nearly impossible to conduct with short reads. In particular, HiFi… read more here.
Keywords: assembled using; rare mutations; hifi; mutations mags ... See more keywords