Multivalent forms of the ribonuclease H1 hybrid binding domain are high‐affinity binders of RNA–DNA hybrids
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14541
Abstract: The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA–DNA hybrids, which are structures present in cellular R‐loops and participate in diverse biological processes. We engineered multivalent HBD… read more here.
Keywords: rna dna; hybrid binding; high affinity; affinity ... See more keywords
Characterisation of an aptamer against the Runt domain of AML1 (RUNX1) by NMR and mutational analyses
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12368
Abstract: Since the invention of systematic evolution of ligands by exponential enrichment, many short oligonucleotides (or aptamers) have been reported that can bind to a wide range of target molecules with high affinity and specificity. Previously,… read more here.
Keywords: high affinity; domain aml1; runt domain; aptamer ... See more keywords
IgE and high‐affinity IgE receptor in chronic inducible urticaria, pathogenic, and management relevance
Sign Up to like & getrecommendations! Published in 2022 at "Clinical and Translational Allergy"
DOI: 10.1002/clt2.12117
Abstract: Abstract Background IgE and high‐affinity IgE receptor (FcεRI) expression on basophils have been scarcely explored in patients with chronic inducible urticaria (CIndU). Objectives To investigate baseline serum IgE and FcεRI expression on blood basophils in… read more here.
Keywords: cindu; high affinity; expression; ige high ... See more keywords
Development of a High‐Affinity Antibody‐Binding Peptide for Site‐Specific Modification
Sign Up to like & getrecommendations! Published in 2021 at "ChemMedChem"
DOI: 10.1002/cmdc.202000977
Abstract: Immunoglobulin G (IgG)‐binding peptides such as 15‐IgBP are convenient tools for the site‐specific modification of antibodies and the preparation of homogeneous antibody–drug conjugates. A peptide such as 15‐IgBP can be selectively crosslinked to the fragment… read more here.
Keywords: high affinity; affinity; site specific; specific modification ... See more keywords
Selection of phage‐displayed antibodies with high affinity and specificity by electrophoresis in microfluidic devices
Sign Up to like & getrecommendations! Published in 2023 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202200187
Abstract: A method development aimed for high‐throughput and automated antibody screening holds great potential for areas ranging from fundamental molecular interactions to the discovery of novel disease markers, therapeutic targets, and monoclonal antibody engineering. Surface display… read more here.
Keywords: high affinity; affinity; phage displayed; selection ... See more keywords
Structural enzymology binding studies of the peptide‐substrate‐binding domain of human collagen prolyl 4‐hydroxylase (type‐II): High affinity peptides have a PxGP sequence motif
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3450
Abstract: The peptide‐substrate‐binding (PSB) domain of collagen prolyl 4‐hydroxylase (C‐P4H, an α2β2 tetramer) binds proline‐rich procollagen peptides. This helical domain (the middle domain of the α subunit) has an important role concerning the substrate binding properties… read more here.
Keywords: substrate binding; high affinity; psb; enzymology ... See more keywords
Dimerization of a ubiquitin variant leads to high affinity interactions with a ubiquitin interacting motif
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.1002/pro.3593
Abstract: We previously described structural and functional characterization of the first ubiquitin variant (UbV), UbV.v27.1, engineered by phage display to bind with high affinity to a specific ubiquitin interacting motif (UIM). We identified two substitutions relative… read more here.
Keywords: interacting motif; high affinity; affinity; dimerization ... See more keywords
Graphene Nanobeacons with High-Affinity Pockets for Combined, Selective, and Effective Decontamination and Reagentless Detection of Heavy Metals.
Sign Up to like & getrecommendations! Published in 2022 at "Small"
DOI: 10.1002/smll.202201003
Abstract: Access to clean water for drinking, sanitation, and irrigation is a major sustainable development goal of the United Nations. Thus, technologies for cleaning water and quality-monitoring must become widely accessible and of low-cost, while being… read more here.
Keywords: high affinity; heavy metals; detection; graphene nanobeacons ... See more keywords
Design and discovery of a high affinity, selective and β-arrestin biased 5-HT7 receptor agonist
Sign Up to like & getrecommendations! Published in 2021 at "Medicinal Chemistry Research"
DOI: 10.1007/s00044-021-02797-4
Abstract: Compound 1c, 5-chloro-2-(2-(3,4-dihydroisoquinolin-2(1H)-yl)ethyl)-2,3-dihydro-1H-inden-1-one was previously reported from our laboratory showing high affinity binding to the 5-HT7 receptor (Ki = 0.5 nM). However, compound 1c racemizes readily upon enantiomeric separation. To prevent racemization, we have redesigned and synthesized methyl… read more here.
Keywords: ht7 receptor; high affinity; arrestin biased;
Behavioural and pharmacological profiles of zebrafish administrated pyrrolidinyl benzodioxanes and prolinol aryl ethers with high affinity for heteromeric nicotinic acetylcholine receptors
Sign Up to like & getrecommendations! Published in 2020 at "Psychopharmacology"
DOI: 10.1007/s00213-020-05536-6
Abstract: Rationale Prolinol aryl ethers and their rigidified analogues pyrrolidinyl benzodioxanes have a high affinity for mammalian α4β2 nicotinic acetylcholine receptors (nAChRs). Electrophysiological studies have shown that the former are full agonists and the latter partial… read more here.
Keywords: aryl ethers; high affinity; prolinol aryl; agonist ... See more keywords
Integrated genomic and transcriptomic insights into the two-component high-affinity nitrate transporters in allotetraploid rapeseed
Sign Up to like & getrecommendations! Published in 2018 at "Plant and Soil"
DOI: 10.1007/s11104-018-3652-3
Abstract: Background and aimsThe two-component high-affinity nitrate (NO3-) transport system (THATS) proteins (NRT2/NAR2) play key roles in the efficient nitrogen (N) uptake and transport under N limitations. We aimed at uncovering the core THATS gene(s) regulating… read more here.
Keywords: high affinity; component high; affinity nitrate; rapeseed ... See more keywords