High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_7
Abstract: High-copy rescue genetic screening is a powerful strategy for the identification of suppression genetic interactions in the model eukaryotic organism Saccharomyces cerevisiae (budding yeast). The strain carrying the mutant allele of interest is transformed with… read more here.
Keywords: saccharomyces cerevisiae; rescue genetic; copy; copy rescue ... See more keywords
Genomic analysis of high copy-number sequences for the targeted detection of Listeria species using a flow-through surveillance system.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of microbiology"
DOI: 10.1007/s00203-021-02388-2
Abstract: The bacterial foodborne pathogen Listeria monocytogenes has been implicated in fresh produce outbreaks with a significant economic impact. Given that L. monocytogenes is widespread in the environment, food production facilities constantly monitor for the presence… read more here.
Keywords: detection; system; listeria; high copy ... See more keywords
High copy number mutants derived from Corynebacterium glutamicum cryptic plasmid pAM330 and copy number control.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2018.10.012
Abstract: A high copy number mutant plasmid, designated pVC7H1, was isolated from an Escherichia coli-Corynebacterium glutamicum shuttle vector pVC7N derived from cryptic plasmid pAM330 that was originally found in Brevibacterium lactofermentum 2256 (formally C. glutamicum ATCC 13869).… read more here.
Keywords: copy number; glutamicum; number; high copy ... See more keywords
High‐copy genome integration and stable production of p‐coumaric acid via a POT1‐mediated strategy in Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Applied Microbiology"
DOI: 10.1111/jam.15593
Abstract: To overcome the defective unstable production of p‐coumaric acid (p‐CA) using episomal plasmids and simultaneously achieve genetic stability and high‐copy integration in Saccharomyces cerevisiae. read more here.
Keywords: coumaric acid; high copy; saccharomyces cerevisiae; production coumaric ... See more keywords
Evolutionary model for the unequal segregation of high copy plasmids
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DOI: 10.1371/journal.pcbi.1006724
Abstract: Plasmids are extrachromosomal DNA elements of microorganisms encoding beneficial genetic information. They were thought to be equally distributed to daughter cells during cell division. Here we use mathematical modeling to investigate the evolutionary stability of… read more here.
Keywords: segregation high; copy plasmids; high copy; segregation ... See more keywords
Characterization of B-Genome Specific High Copy hAT MITE Families in Brassica nigra Genome
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2020.01104
Abstract: Miniature inverted-repeat transposable elements (MITEs) are non-autonomous class II transposons which have been shown to influence genome evolution. Brassica nigra L. (B-genome) is one of three Brassica diploids cultivated primarily as an oil crop, which… read more here.
Keywords: mite families; brassica nigra; high copy; genome ... See more keywords