High-specificity double-stranded DNA detection with a "humanoid" molecular beacon and TALEs.
Sign Up to like & getrecommendations! Published in 2018 at "Nanoscale"
DOI: 10.1039/c8nr05759a
Abstract: Owing to the limitation of DNA-specific recognition, improving the sensitivity of bacteria detection without additional culture poses a considerable challenge. Herein, a sensitive, novel, and high-specificity fluorescence detection method was developed using transcription activator-like effectors… read more here.
Keywords: detection; high specificity; double stranded; stranded dna ... See more keywords
Precise and broad scope genome editing based on high-specificity Cas9 nickases
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa1236
Abstract: Abstract RNA-guided nucleases (RGNs) based on CRISPR systems permit installing short and large edits within eukaryotic genomes. However, precise genome editing is often hindered due to nuclease off-target activities and the multiple-copy character of the… read more here.
Keywords: genome editing; high specificity; specificity cas9; specificity ... See more keywords
Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad165
Abstract: Abstract Homology-directed recombination (HDR) between donor constructs and acceptor genomic sequences cleaved by programmable nucleases, permits installing large genomic edits in mammalian cells in a precise fashion. Yet, next to precise gene knock-ins, programmable nucleases… read more here.
Keywords: crispr cas9; specificity crispr; cas9; high specificity ... See more keywords
Plug-and-play fiber-optic sensors based on engineered cells for neurochemical monitoring at high specificity in freely moving animals.
Sign Up to like & getrecommendations! Published in 2023 at "Science advances"
DOI: 10.1126/sciadv.adg0218
Abstract: In vivo detection of neurochemicals, including neurotransmitters and neuromodulators, is critical for both understanding brain mechanisms and diagnosing brain diseases. However, few sensors are competent in monitoring neurochemical dynamics in vivo at high specificity. Here,… read more here.
Keywords: fiber optic; engineered cells; plug play; based engineered ... See more keywords
OP0304 METABOLOMICS PROFILING OF HUMAN SERUM FOR DISCOVERING BIOMARKERS TO DIAGNOSE PSORIATIC ARTHRITIS AND ANKYLOSING SPONDYLITIS WITH HIGH SPECIFICITY
Sign Up to like & getrecommendations! Published in 2020 at "Annals of the Rheumatic Diseases"
DOI: 10.1136/annrheumdis-2020-eular.5912
Abstract: High-specificity biomarkers that can differentiate PsA and AS patients from healthy people, as well as RA patients, would be extremely helpful for early diagnosis and treatment of these two diseases. Based on the hypothesis that… read more here.
Keywords: serum; none; high specificity; none declared ... See more keywords
Artificial Intelligence Can Cut Costs While Maintaining Accuracy in Colorectal Cancer Genotyping
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2021.630953
Abstract: Rising cancer care costs impose financial burdens on health systems. Applying artificial intelligence to diagnostic algorithms may reduce testing costs and avoid wasteful therapy-related expenditures. To evaluate the financial and clinical impact of incorporating artificial… read more here.
Keywords: intelligence; high specificity; high sensitivity; colorectal cancer ... See more keywords
High-Specificity Test Algorithm for Bovine Tuberculosis Diagnosis in African Buffalo (Syncerus caffer) Herds
Sign Up to like & getrecommendations! Published in 2022 at "Pathogens"
DOI: 10.3390/pathogens11121393
Abstract: Ante-mortem bovine tuberculosis (bTB) tests for buffaloes include the single comparative intradermal tuberculin test (SCITT), interferon-gamma (IFN-γ) release assay (IGRA) and IFN-γ-inducible protein 10 release assay (IPRA). Although parallel test interpretation increases the detection of… read more here.
Keywords: bovis; bovine tuberculosis; test; high specificity ... See more keywords
NEWS2 shows low sensitivity and high specificity for delirium detection: a single site observational study of 13,908 patients.
Sign Up to like & getrecommendations! Published in 2022 at "Clinical medicine"
DOI: 10.7861/clinmed.2022-0345
Abstract: Delirium affects 25% of hospital admissions of older people and is a serious medical condition with poor outcomes. 'New confusion' as a delirium indicator was incorporated into the 'alert, verbal, pain and unresponsive' (AVPU) level… read more here.
Keywords: sensitivity; delirium; high specificity; sensitivity high ... See more keywords