Applying single-cell highly multiplexed secretome proteomics to characterize immunotherapeutic products and predict clinical responses.
Sign Up to like & getrecommendations! Published in 2023 at "Proteomics"
DOI: 10.1002/pmic.202200242
Abstract: Genetically and phenotypically identical immune cell populations can be highly heterogenous in terms of their immune functions and protein secretion profiles. The microfluidic chip-based single-cell highly multiplexed secretome proteomics enables characterization of cellular heterogeneity of… read more here.
Keywords: multiplexed secretome; cell; single cell; cell highly ... See more keywords
Microdroplet PCR for Highly Multiplexed Targeted Bisulfite Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7481-8_17
Abstract: Many methods exist for examining CpG DNA methylation. However, many of these are qualitative, laborious to apply to a large number of genes simultaneously, or are not easy to target to specific regions of interest.… read more here.
Keywords: pcr highly; bisulfite sequencing; highly multiplexed; microdroplet pcr ... See more keywords
Spatial Multiplex In Situ Tagging (MIST) Technology for Rapid, Highly Multiplexed Detection of Protein Distribution on Brain Tissue.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c04970
Abstract: Highly multiplexed analysis of biospecimens significantly advances the understanding of biological basics of diseases, but these techniques are limited by the number of multiplexity and the speed of processing. Here, we present a rapid multiplex… read more here.
Keywords: highly multiplexed; multiplex; detection; technology ... See more keywords
Highly multiplexed rapid DNA detection with single-nucleotide specificity via convective PCR in a portable device.
Sign Up to like & getrecommendations! Published in 2021 at "Nature biomedical engineering"
DOI: 10.1038/s41551-021-00755-4
Abstract: Assays for the molecular detection of nucleic acids are typically constrained by the level of multiplexing (this is the case for the quantitative polymerase chain reaction (qPCR) and for isothermal amplification), turnaround times (as with… read more here.
Keywords: detection; dna; single nucleotide; highly multiplexed ... See more keywords
Highly multiplexed single-cell in situ RNA and DNA analysis with bioorthogonal cleavable fluorescent oligonucleotides† †Electronic supplementary information (ESI) available: Additional figures, schemes, probe sequences and experimental details. See DOI: 10.1039/c7sc05089e
Sign Up to like & getrecommendations! Published in 2018 at "Chemical Science"
DOI: 10.1039/c7sc05089e
Abstract: Bioorthogonal cleavable fluorescent oligonucleotides have been developed to enable highly multiplexed single-cell in situ RNA and DNA analysis. read more here.
Keywords: single cell; fluorescent oligonucleotides; multiplexed single; highly multiplexed ... See more keywords
Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies
Sign Up to like & getrecommendations! Published in 2018 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1721899115
Abstract: Significance Next-generation sequencing (NGS) has allowed the comprehensive study of the genome and transcriptome. However, a similarly broad, highly multiplexed, and inexpensive method for proteomics using NGS remains elusive. Here, we describe a phage display-based… read more here.
Keywords: using genetically; surface; highly multiplexed; cell surface ... See more keywords
Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries
Sign Up to like & getrecommendations! Published in 2019 at "Synthetic Biology"
DOI: 10.1093/synbio/ysz025
Abstract: Abstract Synthetic biology utilizes the Design–Build–Test–Learn pipeline for the engineering of biological systems. Typically, this requires the construction of specifically designed, large and complex DNA assemblies. The availability of cheap DNA synthesis and automation enables… read more here.
Keywords: fast accurate; dna; analysis; biology ... See more keywords
Oligonucleotide conjugated antibodies permit highly multiplexed immunofluorescence for future use in clinical histopathology
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biomedical Optics"
DOI: 10.1117/1.jbo.25.5.056004
Abstract: Abstract. Significance: Advanced genetic characterization has informed cancer heterogeneity and the challenge it poses to effective therapy; however, current methods lack spatial context, which is vital to successful cancer therapy. Conventional immunolabeling, commonplace in the… read more here.
Keywords: future use; use clinical; highly multiplexed; conjugated antibodies ... See more keywords
43 Highly multiplexed digital spatial profiling of the tumor microenvironment of non-small-cell lung cancer (NSCLC)
Sign Up to like & getrecommendations! Published in 2020 at "Journal for ImmunoTherapy of Cancer"
DOI: 10.1136/jitc-2020-sitc2020.0043
Abstract: Background Profiling the tumour microenvironment (TME) has been informative in understanding the underlying tumour-immune interactions. Multiplex immunohistochemistry(mIHC) coupled with molecular barcoding technologies have revealed greater insights into the TME. Methods In this study, we utilised… read more here.
Keywords: microenvironment; digital spatial; multiplexed digital; highly multiplexed ... See more keywords
Abstract 2089: Highly multiplexed analysis of immune cell subsets in non-small cell lung cancer: validation of protein and RNA analysis by the Nanostring Digital Spatial Profiling (DSP) platform
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-2089
Abstract: Background: Highly multiplexed spatial analysis of tumor tissue is necessary to improve understanding of anti-tumor immunity. Moreover, spatial interrogation of protein and nucleic acid targets simultaneously can better characterize immune cell subsets and targets such… read more here.
Keywords: immune cell; analysis; cell subsets; highly multiplexed ... See more keywords
Abstract P5-02-03: Highly multiplexed tissue-based cyclic immunofluorescence in breast cancer for precision oncology
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.sabcs19-p5-02-03
Abstract: Background: Tissue-based cyclic immunofluorescence (t-CyCIF) is a recently described technique for highly multiplexed immunofluorescence microscopy of formalin-fixed, paraffin-embedded (FFPE) specimens. Via an iterative process, successive four-channel images are collected from the same sample and then… read more here.
Keywords: breast cancer; tissue; highly multiplexed; cancer ... See more keywords