Endotoxin modulates the electrophysiological characteristics of human embryonic stem cell‐differentiated cardiomyocytes
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.27251
Abstract: Gram‐negative bacteria‐induced infections result in fever, arrhythmia, and even death. Lipopolysaccharide (LPS), a constituent of bacteria, leads to an inflammatory response under sepsis and increase arrhythmogenesis. This study analyzed the effects on human embryonic stem… read more here.
Keywords: channel current; channel; embryonic stem; cell ... See more keywords
Pneumatic unidirectional cell stretching device for mechanobiological studies of cardiomyocytes
Sign Up to like & getrecommendations! Published in 2019 at "Biomechanics and Modeling in Mechanobiology"
DOI: 10.1007/s10237-019-01211-8
Abstract: In this paper, we present a transparent mechanical stimulation device capable of uniaxial stimulation, which is compatible with standard bioanalytical methods used in cellular mechanobiology. We validate the functionality of the uniaxial stimulation system using… read more here.
Keywords: microscopy; stretching device; pneumatic unidirectional; cell ... See more keywords
Machine-learning-based quality control of contractility of cultured human-induced pluripotent stem-cell-derived cardiomyocytes.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.03.141
Abstract: The precise and early assessment of cardiotoxicity is fundamental to bring forward novel drug candidates to the pharmaceutical market and to avoid their withdrawal from the market. Recent preclinical studies have attempted to use human-induced… read more here.
Keywords: human induced; pluripotent stem; induced pluripotent; contractility ... See more keywords
Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.09.021
Abstract: Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) resemble fetal cardiomyocytes and electrical stimulation (ES) has been explored to mature the differentiated cells. Here, we hypothesize that ES applied at the beginning of the differentiation… read more here.
Keywords: cardiac conduction; conduction like; electrical stimulation; differentiation ... See more keywords
Myogenin suppresses apoptosis induced by angiotensin II in human induced pluripotent stem cell-derived cardiomyocytes.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.03.031
Abstract: BACKGROUND Angiotensin II (Ang II), an important component of the renin-angiotensin system (RAS), plays a critical role in the pathogenesis of cardiovascular disorders. In addition, human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been considered… read more here.
Keywords: apoptosis; human induced; induced pluripotent; ang ... See more keywords
Phenotypic variability in LQT3 human induced pluripotent stem cell-derived cardiomyocytes and their response to antiarrhythmic pharmacologic therapy: An in silico approach
Sign Up to like & getrecommendations! Published in 2017 at "Heart Rhythm"
DOI: 10.1016/j.hrthm.2017.07.026
Abstract: Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are in vitro models with the clear advantages of their human origin and suitability for human disease investigations. However, limitations include their incomplete characterization and variability reported… read more here.
Keywords: phenotypic variability; variability lqt3; hipsc cms; variability ... See more keywords
Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes
Sign Up to like & getrecommendations! Published in 2018 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2018.01.012
Abstract: Summary Human induced pluripotent stem cell (hiPSC) cardiomyocytes (CMs) show less negative resting membrane potential (RMP), which is attributed to small inward rectifier currents (IK1). Here, IK1 was measured in hiPSC-CMs (proprietary and commercial cell… read more here.
Keywords: cms; membrane potential; resting membrane; hipsc cms ... See more keywords
Development of a Human iPSC Cardiomyocyte-Based Scoring System for Cardiac Hazard Identification in Early Drug Safety De-risking
Sign Up to like & getrecommendations! Published in 2018 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2018.11.007
Abstract: Summary Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a promising cardiac safety platform, demonstrated by numerous validation studies using drugs with known cardiac adverse effects in humans. However, the challenge remains to… read more here.
Keywords: hazard; cardiac hazard; system cardiac; safety ... See more keywords
Increased tissue stiffness triggers contractile dysfunction and telomere shortening in dystrophic cardiomyocytes
Sign Up to like & getrecommendations! Published in 2021 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2021.04.018
Abstract: Summary Duchenne muscular dystrophy (DMD) is a rare X-linked recessive disease that is associated with severe progressive muscle degeneration culminating in death due to cardiorespiratory failure. We previously observed an unexpected proliferation-independent telomere shortening in… read more here.
Keywords: dmd; telomere shortening; tissue stiffness; stiffness triggers ... See more keywords
Cell culture conditions affect the ability of high content imaging assay to detect drug-induced changes in cellular parameters in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs)
Sign Up to like & getrecommendations! Published in 2019 at "Toxicology Reports"
DOI: 10.1016/j.toxrep.2019.02.004
Abstract: Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are widely used for drug safety and efficacy testing with various techniques, including high content imaging (HCI). Upon drug treatment, a significant number of hiPSC-CMs grown in regular… read more here.
Keywords: high content; content imaging; drug; cell ... See more keywords
The assessment of electrophysiological activity in human-induced pluripotent stem cell-derived cardiomyocytes exposed to dimethyl sulfoxide and ethanol by manual patch clamp and multi-electrode array system.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of pharmacological and toxicological methods"
DOI: 10.1016/j.vascn.2017.03.003
Abstract: INTRODUCTION Recently, electrophysiological activity has been effectively measured in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to predict drug-induced arrhythmia. Dimethyl sulfoxide (DMSO) and ethanol have been used as diluting agents in many experiments. However, the… read more here.
Keywords: dmso ethanol; patch clamp; cell; hipsc cms ... See more keywords