A novel nickel-modified nano-magnetite for isolation of histidine-tagged proteins expressed in Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03637-5
Abstract: Nano-magnetite with superparamagnetism could be coated by some organic compounds or by nano Au or Pt via surface modifications with multi-step reactions for the applications of isolating histidine-tagged (His-tagged) proteins. Introducing active sites of binding… read more here.
Keywords: nano magnetite; escherichia coli; surface; histidine tagged ... See more keywords
Detection of Histidine-Tagged Protein in Escherichia coli by Single-Cell Inductively Coupled Plasma-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00774
Abstract: We have developed a rapid and precise quantification method for a histidine (His)-tagged recombinant protein produced in Escherichia coli (E. coli) by single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). Plasmid vector containing enhanced green fluorescent protein… read more here.
Keywords: escherichia coli; single cell; mcherry; coli single ... See more keywords
Droplet-Based Immunosensor for Simultaneous Immunoassays of Multiplex Histidine-Tagged Proteins
Sign Up to like & getrecommendations! Published in 2019 at "SLAS Technology"
DOI: 10.1177/2472630319879647
Abstract: This paper presents a droplet-based immunoassay chip allowing each droplet to be positioned in a passive droplet-positioning cavern under continuous flow. In addition, the chip surface can immobilize any kind of histidine-tagged capture agents for… read more here.
Keywords: multiplex histidine; histidine; histidine tagged; droplet based ... See more keywords