Distinguishing Histidine Tautomers in Proteins Using Covalent Labeling-Mass Spectrometry.
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DOI: 10.1021/acs.analchem.1c03902
Abstract: In this work, we use diethylpyrocarbonate (DEPC)-based covalent labeling together with LC-MS/MS analysis to distinguish the two sidechain tautomers of histidine residues in peptides and proteins. From labeling experiments on model peptides, we demonstrate that… read more here.
Keywords: histidine residues; tautomers proteins; covalent labeling; histidine tautomers ... See more keywords