Holin‐assisted bacterial recombinant protein export
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28179
Abstract: A simple generic method for enhancing extracellular protein yields in engineered bacteria is still lacking. Here, we demonstrated that phage‐encoded holin can be used to export proteins to the extracellular medium in both Gram‐negative Escherichia… read more here.
Keywords: hol380; holin assisted; holin; lactis ... See more keywords
Getting Outside the Cell: Versatile Holin Strategies Used by Distinct Phages to Leave Their Bacillus thuringiensis Host
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.00696-22
Abstract: The phage life cycle ends with the host cell lysis, thereby releasing new virions into the environment for the next round of bacterial infection. Nowadays, there is renewed interest in phages as biocontrol agents, primarily… read more here.
Keywords: cell; bacillus thuringiensis; lysis; holin ... See more keywords
Deciphering the Role of Holin in Mycobacteriophage D29 Physiology
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2020.00883
Abstract: In the era of antibiotic resistance, phage therapy is gaining attention for the treatment of pathogenic organisms such as Mycobacterium tuberculosis. The selection of phages for therapeutic purposes depends upon several factors such as the… read more here.
Keywords: phage; cell lysis; d29; host cell ... See more keywords
Biological Characterization and Evolution of Bacteriophage T7-△holin During the Serial Passage Process
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DOI: 10.3389/fmicb.2021.705310
Abstract: Bacteriophage T7 gene 17.5 coding for the only known holin is one of the components of its lysis system, but the holin activity in T7 is more complex than a single gene, and evidence points… read more here.
Keywords: microscopy; serial passage; lysis; gene ... See more keywords
Characterization of a lytic Pseudomonas aeruginosa phage vB_PaeP_ASP23 and functional analysis of its lysin LysASP and holin HolASP
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DOI: 10.3389/fmicb.2023.1093668
Abstract: In this study, we isolated a lytic Pseudomonas aeruginosa phage (vB_PaeP_ASP23) from the sewage of a mink farm, characterized its complete genome and analyzed the function of its putative lysin and holin. Morphological characterization and… read more here.
Keywords: lytic pseudomonas; phage asp23; aeruginosa phage; pseudomonas aeruginosa ... See more keywords
Functional Dissection of P1 Bacteriophage Holin-like Proteins Reveals the Biological Sense of P1 Lytic System Complexity
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23084231
Abstract: P1 is a model temperate myovirus. It infects different Enterobacteriaceae and can develop lytically or form lysogens. Only some P1 adaptation strategies to propagate in different hosts are known. An atypical feature of P1 is… read more here.
Keywords: lytic system; sar endolysin; cell lysis; holin ... See more keywords