Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00340
Abstract: Prokaryote genomes encode diverse programmable DNA endonucleases with significant potential for biotechnology and gene editing. However, these endonucleases differ significantly in their properties, which must be screened and measured. While positive selection screens based on… read more here.
Keywords: homing endonuclease; endonuclease scei; gene editing; positive selection ... See more keywords
The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.856981
Abstract: Making discrete and precise genetic changes to wild populations has been proposed as a means of addressing some of the world’s most pressing ecological and public health challenges caused by insect pests. Technologies that would… read more here.
Keywords: challenges developing; developing efficient; endonuclease gene; gene ... See more keywords
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.855511
Abstract: Inteins catalyze their removal from a host protein through protein splicing. Inteins that contain an additional site-specific endonuclease domain display genetic mobility via a process termed “homing” and thereby act as selfish DNA elements. We… read more here.
Keywords: homing endonuclease; endonuclease domain; basis propagation; endonuclease ... See more keywords