Homo-FRET Imaging to Study Protein-Protein Interaction and Complex Formation in Plants.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1791-5_12
Abstract: Protein-protein interactions in living plant cells can be measured by changes in fluorescence anisotropy due to homo-FRET (Förster Resonance Energy Transfer). Here, the energy transfer between identical fluorophores, e.g., enhanced green fluorescent protein (EGFP) fused… read more here.
Keywords: complex formation; protein protein; homo fret; fret imaging ... See more keywords
Fluorescence Anisotropy as a Self-Referencing Readout for Ion-Selective Sensing and Imaging Using Homo-FRET between Chromoionophores.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01532
Abstract: Fluorescence anisotropy has been widely used in developing biosensors and immunoassays, by virtue of the self-reference and environment-sensitive properties. However, fluorescence anisotropic chemical sensors on inorganic ions are limited by the total anisotropy change. To… read more here.
Keywords: fluorescence anisotropy; homo fret; fluorescence; ion selective ... See more keywords
Homo-FRET in π-Conjugated Polygons: Intermediate-Strength Dipole-Dipole Coupling Makes Energy Transfer Reversible.
Sign Up to like & getrecommendations! Published in 2019 at "Nano letters"
DOI: 10.1021/acs.nanolett.9b01998
Abstract: The concept of homo-FRET is often used to describe energy transfer between like chromophores of molecular aggregates such as in π-conjugated polymers. Homo-FRET is revealed by a dynamic depolarization in fluorescence, but strictly only applies… read more here.
Keywords: energy transfer; homo fret; energy; dipole coupling ... See more keywords
Probing the Structural Dynamics of the Activation Gate of KcsA Using Homo-FRET Measurements
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms222111954
Abstract: The allosteric coupling between activation and inactivation processes is a common feature observed in K+ channels. Particularly, in the prokaryotic KcsA channel the K+ conduction process is controlled by the inner gate, which is activated… read more here.
Keywords: homo fret; homo; gate; fret measurements ... See more keywords