Accurate profiling of full-length Fv in highly homologous antibody libraries using UMI tagged short reads.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad235
Abstract: Deep parallel sequencing (NGS) is a viable tool for monitoring scFv and Fab library dynamics in many antibody engineering high-throughput screening efforts. Although very useful, the commonly used Illumina NGS platform cannot handle the entire… read more here.
Keywords: full length; antibody libraries; homologous antibody; highly homologous ... See more keywords