MND1 enables homologous recombination in somatic cells primarily outside the context of replication.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular oncology"
DOI: 10.1002/1878-0261.13448
Abstract: Faithful and timely repair of DNA double-strand breaks (DSBs) is fundamental for the maintenance of genomic integrity. Here, we demonstrate that the meiotic recombination co-factor MND1 facilitates the repair of DSBs in somatic cells. We… read more here.
Keywords: recombination; mnd1 enables; replication; mnd1 ... See more keywords
CRISPRi repression of nonhomologous end‐joining for enhanced genome engineering via homologous recombination in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26404
Abstract: In many organisms of biotechnological importance precise genome editing is limited by inherently low homologous recombination (HR) efficiencies. A number of strategies exist to increase the effectiveness of this native DNA repair pathway; however, most… read more here.
Keywords: homologous recombination; repression; nonhomologous end; crispri ... See more keywords
The emerging role of homologous recombination repair and PARP inhibitors in genitourinary malignancies
Sign Up to like & getrecommendations! Published in 2017 at "Cancer"
DOI: 10.1002/cncr.30631
Abstract: As cells age and are exposed to genotoxic stress, preservation of the genomic code requires multiple DNA repair pathways to remove single‐strand or double‐strand breaks. Loss of function somatic genomic aberrations or germline deficiency in… read more here.
Keywords: parp inhibitors; genitourinary malignancies; homologous recombination; strand ... See more keywords
Homologous recombination repair deficiency (HRD): From biology to clinical exploitation
Sign Up to like & getrecommendations! Published in 2021 at "Genes"
DOI: 10.1002/gcc.22939
Abstract: Significant advances in cancer precision medicine over the last decade have led to a number of treatment options that have significantly improved patient outcomes. The underlying concept integrates detailed insights into disease mechanisms with clinical… read more here.
Keywords: hrd; homologous recombination; biology; recombination repair ... See more keywords
A simple approach for random genomic insertion–deletions using ambiguous sequences in Escherichia coli
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202100636
Abstract: Escherichia coli K‐12, being one of the best understood and thoroughly analyzed organisms, is the preferred platform for genetic and biochemical research. Among all genetic engineering approaches applied on E. coli, the homologous recombination approach… read more here.
Keywords: escherichia coli; ambiguous sequences; insertion deletions; insertion ... See more keywords
Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7339-2_15
Abstract: Tagging of genes at the endogenous loci is a powerful strategy for the analysis of protein function. We have developed a homologous recombination-based approach for inserting epitope tag into Drosophila AGO1 locus by employing the… read more here.
Keywords: ago1; crispr cas9; homologous recombination; genomic tagging ... See more keywords
Gene Replacement by Homologous Recombination.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9210-2_8
Abstract: While homologous recombination-based gene replacement is about to be supplanted by more modern approaches, it is still retaining usefulness for genes that prove to be poor targets for CRISPR/cas-based approaches. Homologous recombination has proven to… read more here.
Keywords: homologous recombination; gene replacement; replacement homologous; recombination ... See more keywords
Structural basis of homologous recombination
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-019-03365-1
Abstract: Homologous recombination (HR) is a pathway to faithfully repair DNA double-strand breaks (DSBs). At the core of this pathway is a DNA recombinase, which, as a nucleoprotein filament on ssDNA, pairs with homologous DNA as… read more here.
Keywords: structural basis; dna; microscopy; homologous recombination ... See more keywords
Homologous recombination deficiency in epithelial ovarian cancer
Sign Up to like & getrecommendations! Published in 2020 at "memo - Magazine of European Medical Oncology"
DOI: 10.1007/s12254-020-00606-z
Abstract: Since the introduction of poly-ADP-ribose polymerase (PARP) inhibitor therapy for epithelial ovarian cancer (EOC) patients, testing for aberrations of homologous recombination (HR) repair as a predictive biomarker of therapy response has become an area of… read more here.
Keywords: therapy; homologous recombination; epithelial ovarian; ovarian cancer ... See more keywords
Homologous recombination deficiency in triple negative breast cancer.
Sign Up to like & getrecommendations! Published in 2019 at "Breast"
DOI: 10.1016/j.breast.2019.02.007
Abstract: Triple negative breast cancer (TNBC) represents a heterogeneous subtype of breast cancer characterized by an unfavorable prognosis due to its aggressive biology. The median overall survival (OS) for patients with metastatic TNBC is around 9-12… read more here.
Keywords: homologous recombination; triple negative; breast cancer;
UBQLN4 Represses Homologous Recombination and Is Overexpressed in Aggressive Tumors
Sign Up to like & getrecommendations! Published in 2019 at "Cell"
DOI: 10.1016/j.cell.2018.11.024
Abstract: Genomic instability can be a hallmark of both human genetic disease and cancer. We identify a deleterious UBQLN4 mutation in families with an autosomal recessive syndrome reminiscent of genome instability disorders. UBQLN4 deficiency leads to increased sensitivity to… read more here.
Keywords: tumors ubqln4; homologous recombination; ubqln4 represses; aggressive tumors ... See more keywords