Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00366
Abstract: There is a growing interest in establishing the methylotrophic yeast Pichia pastoris as microbial cell factories for producing fuels, chemicals, and natural products, particularly with methanol as the feedstock. Although CRISPR (Clustered Regularly Interspaced Short… read more here.
Keywords: recombination; integration; homology arms; efficiency ... See more keywords
An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms
Sign Up to like & getrecommendations! Published in 2019 at "Nature chemical biology"
DOI: 10.1038/s41589-019-0432-1
Abstract: Here, we report a rapid CRISPR–Cas9-mediated gene knock-in strategy that uses Cas9 ribonucleoprotein and 5′-modified double-stranded DNA donors with 50-base-pair homology arms and achieved unprecedented 65/40% knock-in rates for 0.7/2.5 kilobase inserts, respectively, in human embryonic… read more here.
Keywords: strategy; gene knock; gene; homology arms ... See more keywords
An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms
Sign Up to like & getrecommendations! Published in 2019 at "eLife"
DOI: 10.7554/elife.51539
Abstract: We previously reported a CRISPR-mediated knock-in strategy into introns of Drosophila genes, generating an attP-FRT-SA-T2A-GAL4-polyA-3XP3-EGFP-FRT-attP transgenic library for multiple uses (Lee et al., 2018a). The method relied on double stranded DNA (dsDNA) homology donors with… read more here.
Keywords: homology arms; crispr based; based strategy; efficient crispr ... See more keywords