Adenovirus in the omics era – a multipronged strategy
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13710
Abstract: Human adenoviruses (HAdVs) are common pathogens associated with a wide variety of respiratory, ocular, and gastrointestinal diseases. To achieve its effective lytic mode of replication, HAdVs have to reprogram host‐cell gene expression and fine‐tune viral… read more here.
Keywords: era multipronged; omics era; multipronged strategy; host cell ... See more keywords
Characterisation of the E. coli HMS174 and BLR host cell proteome to guide purification process development.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology journal"
DOI: 10.1002/biot.202300068
Abstract: Mass-spectrometry-based proteomics is increasingly employed to monitor purification processes or to detect critical host cell proteins in the final drug substance. This approach is inherently unbiased and can be used to identify individual host cell… read more here.
Keywords: purification; host cell; process development;
Comparison of platform host cell protein ELISA to process‐specific host cell protein ELISA
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26466
Abstract: During expression of biotherapeutic proteins, complex mixtures of additional proteins are also produced by normal expression machinery of the host cell (termed “host cell proteins,” or HCP). HCPs pose a potential impact to patient safety… read more here.
Keywords: hcp; process; host cell; process specific ... See more keywords
Comparison of purification strategies for antibodies used in a broad spectrum host cell protein immunoassay
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26482
Abstract: Host cell proteins (HCPs) are a heterogeneous mixture of impurities that should be minimized in bulk preparations of biotechnologically produced medicines. Immunoassays are commonly used to detect and measure HCPs in therapeutic products, and a… read more here.
Keywords: host cell; purification; comparison purification; purification strategies ... See more keywords
Holistic analytical characterization and risk assessment of residual host cell protein impurities in an active pharmaceutical ingredient synthesized by biocatalysts
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28112
Abstract: Host cell proteins (HCPs) are a significant class of process‐related impurities commonly associated with the manufacturing of biopharmaceuticals. However, due to the increased use of crude enzymes as biocatalysts for modern organic synthesis, HCPs can… read more here.
Keywords: cell; active pharmaceutical; risk assessment; host cell ... See more keywords
The agony of choice: Impact of the host animal species on the enzyme‐linked immunosorbent assay performance for host cell protein quantification
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28265
Abstract: Host cell proteins (HCPs) are inevitable process‐related impurities in biotherapeutics commonly monitored by enzyme‐linked immunosorbent assays (ELISAs). Of particular importance for their reliable detection are the anti‐HCP polyclonal antibodies (pAbs), supposed to detect a broad… read more here.
Keywords: enzyme linked; host animal; linked immunosorbent; host ... See more keywords
Screening different host cell lines for the dynamic production of measles virus
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Progress"
DOI: 10.1002/btpr.2432
Abstract: Measles virus (MV) has a natural affinity for cancer cells and oncolytic MV preparations have therefore been investigated in several clinical trials as a potential treatment for cancer. The main bottleneck in the administration of… read more here.
Keywords: production; different host; host cell; host ... See more keywords
Experience with host cell protein impurities in biopharmaceuticals
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2640
Abstract: In the 40‐year history of biopharmaceuticals, there have been a few cases where the final products contained residual host cell protein (HCP) impurities at levels high enough to be of concern. This article summarizes the… read more here.
Keywords: host cell; cell protein; hcp impurities; experience ... See more keywords
Enhancing Protein A performance in mAb processing: A method to reduce and rapidly evaluate host cell DNA levels during primary clarification
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2882
Abstract: The use and impact of 3M™ Emphaze™ AEX Hybrid Purifier, a single‐use, fully synthetic chromatographic product, was explored to reduce host cell DNA (HC‐DNA) concentration during the primary clarification of a monoclonal antibody (mAb). An… read more here.
Keywords: clarification; host cell; primary clarification; dna ... See more keywords
Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3254
Abstract: In recent years, targeted gene integration (TI) has been introduced as a strategy for the generation of recombinant mammalian cell lines for the production of biotherapeutics. Besides reducing the immense heterogeneity within a pool of… read more here.
Keywords: site specific; cell; host cell; specific integration ... See more keywords
Analytical characterization of host-cell-protein-rich aggregates in monoclonal antibody solutions.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3343
Abstract: Host-cell proteins (HCPs) and high molecular weight (HMW) species have historically been treated as independent classes of impurities in the downstream processing of monoclonal antibodies (mAbs), but recent indications suggest that they may be partially… read more here.
Keywords: cell; hccf protein; protein eluate; host cell ... See more keywords