An Alternative Hot Start PCR Method Using a Nuclease-Deficient ExoIII from Escherichia coli
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DOI: 10.1007/s12033-019-00216-z
Abstract: The Hot Start polymerase chain reaction (Hot Start PCR) is designed to reduce off-target amplification by blocking DNA polymerase extension at room temperature until the desired temperature is reached. In this study, we investigated a… read more here.
Keywords: start; temperature; escherichia coli; hot start ... See more keywords