Novel HPLC-Based Screening Method to Assess Developability of Antibody-Like Molecules.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6857-2_11
Abstract: The discovery of antibodies that bind to targets with high affinity is now a routine exercise. However, it is still challenging to screen for candidates that, in addition to having excellent biological properties, also have… read more here.
Keywords: method assess; hplc based; assess developability; based screening ... See more keywords
A combined NMR- and HPLC-MS/MS-based metabolomics to evaluate the metabolic perturbations and subacute toxic effects of endosulfan on mice
Sign Up to like & getrecommendations! Published in 2017 at "Environmental Science and Pollution Research"
DOI: 10.1007/s11356-017-9534-z
Abstract: Endosulfan is the newly persistent organic pollutants (POPs) added to the Stockholm Convention as its widespread use, persistence, bioaccumulation, long-range transport, endocrine disruption, and toxicity related to various adverse effects. In the present study, male… read more here.
Keywords: metabolism; perturbations subacute; endosulfan; metabolic perturbations ... See more keywords
HPLC-based kinetics assay facilitates analysis of systems with multiple reaction products and thermal enzyme denaturation.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2016.10.010
Abstract: Glucosinolates are plant secondary metabolites abundant in Brassica vegetables that are substrates for the enzyme myrosinase, a thioglucoside hydrolase. Enzyme-mediated hydrolysis of glucosinolates forms several organic products, including isothiocyanates (ITCs) that have been explored for… read more here.
Keywords: denaturation; multiple reaction; non natural; hplc based ... See more keywords
A practical method for preparing fluorescent-labeled glycans with a 9-fluorenylmethyl derivative to simplify a fluorimetric HPLC-based analysis.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113267
Abstract: Analysis of glycans in glycoproteins is often performed by liquid chromatography (LC) separation coupled with fluorescence detection and/or mass spectrometric detection. Enzymatically or chemically released glycans from glycoproteins are usually labeled by reductive amination with… read more here.
Keywords: fluorescent labeled; labeled glycans; analysis; method ... See more keywords
A highly sensitive and selective high pressure liquid chromatography with tandem mass spectrometry (HPLC/MS-MS) method for the direct peptide reactivity assay (DPRA).
Sign Up to like & getrecommendations! Published in 2018 at "Journal of pharmacological and toxicological methods"
DOI: 10.1016/j.vascn.2018.07.004
Abstract: While the HPLC/UV (high performance liquid chromatography coupled with ultra-violet spectrometry)-based DPRA (Direct Peptide Reactivity Assay) identifies dermal sensitizers with approximately 80% accuracy, the low selectivity and sensitivity of the HPLC/UV-based DPRA poses challenges to… read more here.
Keywords: based dpra; liquid chromatography; hplc based;
HPLC based single-step kinetic assay to screen the activity of DNAzymes
Sign Up to like & getrecommendations! Published in 2017 at "Analytical Methods"
DOI: 10.1039/c6ay03061k
Abstract: DNAzymes are a new class of biosensors and pharmaceuticals whose mode-of-action is to cleave RNA. It is important to determine their cleavage rate during discovery and optimization as well as in quality control. We developed… read more here.
Keywords: step; single step; kinetic assay; hplc based ... See more keywords
A critical review of HPLC-based analytical methods for quantification of Linezolid
Sign Up to like & getrecommendations! Published in 2019 at "Critical Reviews in Analytical Chemistry"
DOI: 10.1080/10408347.2019.1605876
Abstract: Abstract Linezolid is a synthetic antimicrobial agent belonging to the oxazolidinone class. Since its approval in the year 2000 until now, linezolid remains the main representative drug for the oxazolidinone class of drugs, which is… read more here.
Keywords: analytical methods; review; quantification linezolid; hplc based ... See more keywords
HPLC-Based Activity Profiling for Antiprotozoal Compounds in Croton gratissimus and Cuscuta hyalina
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2020.01246
Abstract: In a screening of Sudanese medicinal plants for antiprotozoal activity, the chloroform fractions obtained by liquid-liquid partitioning from ethanolic extracts of fruits of Croton gratissimus var. gratissimus and stems of Cuscuta hyalina Roth ex Schult.… read more here.
Keywords: based activity; hplc based; cuscuta hyalina; croton gratissimus ... See more keywords