Identification and Characterization of the 28-N-Methyltransferase Involved in HSAF Analogue Biosynthesis.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00575
Abstract: Polycyclic tetramate macrolactams (PoTeMs) are a family of structurally intriguing bioactive natural products. Although the presence of the N-28 methyl group is known to affect bioactivities of some PoTeMs, the mechanism for this methylation remains… read more here.
Keywords: characterization methyltransferase; identification characterization; methyltransferase; hsaf ... See more keywords
Outer Membrane Vesicle-Mediated Codelivery of the Antifungal HSAF Metabolites and Lytic Polysaccharide Monooxygenase in the Predatory Lysobacter enzymogenes.
Sign Up to like & getrecommendations! Published in 2021 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00260
Abstract: Lysobacter are new biocontrol agents known for their prolific production of lytic enzymes and bioactive metabolites. L. enzymogenes is a predator of fungi and produces several structurally distinct antimicrobial compounds, such as the antifungal HSAF… read more here.
Keywords: antifungal hsaf; polysaccharide monooxygenase; outer membrane; hsaf ... See more keywords
Construction of a hybrid gene cluster to reveal coupled ring formation-hydroxylation in the biosynthesis of HSAF and analogues from Lysobacter enzymogenes.
Sign Up to like & getrecommendations! Published in 2019 at "MedChemComm"
DOI: 10.1039/c9md00154a
Abstract: HSAF and analogues are polycyclic tetramate macrolactams (PoTeMs) isolated from Lysobacter enzymogenes. Due to their antifungal activity, distinct chemical structure and new mode of action, PoTeMs have been the subject of several studies for their… read more here.
Keywords: ring formation; formation; cluster; hsaf ... See more keywords
Optimization of culture conditions for promoting heat-stable antifungal factor production level in Lysobacter enzymogenes.
Sign Up to like & getrecommendations! Published in 2019 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnz007
Abstract: Heat-stable antifungal factor (HSAF) was firstly isolated from Lysobacter enzymogenes, it occupies inhibitory activities against a wide range of pathogens, but a low level of HSAF was obtained from L. enzymogenes cultured in 0.1 × tryptic soy… read more here.
Keywords: heat stable; stable antifungal; antifungal factor; level ... See more keywords
4‐Hydroxybenzoic acid is a diffusible factor that connects metabolic shikimate pathway to the biosynthesis of a unique antifungal metabolite in Lysobacter enzymogenes
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Microbiology"
DOI: 10.1111/mmi.13619
Abstract: Heat‐stable antifungal factor (HSAF) produced by Lysobacter enzymogenes is a potential lead compound for developing new antibiotics. Yet, how L. enzymogenes regulates the HSAF biosynthesis remains largely unknown. Here, we show that 4‐hydroxybenzoic acid (4‐HBA)… read more here.
Keywords: biosynthesis; production; shikimate pathway; hsaf ... See more keywords
Heat-Stable Antifungal Factor (HSAF) Biosynthesis in Lysobacter enzymogenes Is Controlled by the Interplay of Two Transcription Factors and a Diffusible Molecule
Sign Up to like & getrecommendations! Published in 2017 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01754-17
Abstract: ABSTRACT Lysobacter enzymogenes is a Gram-negative, environmentally ubiquitous bacterium that produces a secondary metabolite, called heat-stable antifungal factor (HSAF), as an antifungal factor against plant and animal fungal pathogens. 4-Hydroxybenzoic acid (4-HBA) is a newly… read more here.
Keywords: hsaf biosynthesis; molecule; hsaf; hba ... See more keywords
Separation of Heat-Stable Antifungal Factor From Lysobacter enzymogenes Fermentation Broth via Photodegradation and Macroporous Resin Adsorption
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.663065
Abstract: Heat-stable antifungal factor (HSAF) is produced by the fermentation of Lysobacter enzymogenes, which is known for its broad-spectrum antifungal activity and novel mode of action. However, studies on the separation of HSAF have rarely been… read more here.
Keywords: resin; adsorption; heat stable; photodegradation ... See more keywords
LeTetR Positively Regulates 3-Hydroxylation of the Antifungal HSAF and Its Analogs in Lysobacter enzymogenes OH11
Sign Up to like & getrecommendations! Published in 2020 at "Molecules"
DOI: 10.3390/molecules25102286
Abstract: The biocontrol agent Lysobacter enzymogenes OH11 produces several structurally distinct antibiotic compounds, including the antifungal HSAF (Heat Stable Antifungal Factor) and alteramides, along with their 3-dehydroxyl precursors (3-deOH). We previously showed that the 3-hydroxylation is… read more here.
Keywords: antifungal hsaf; hydroxylation; enzymogenes oh11; regulates hydroxylation ... See more keywords