The Use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7896-0_27
Abstract: The CRISPR-Cas9 technology is a powerful tool that enables site-specific genome modification (gene editing) and is increasingly used in research to generate gene knockout or knock-in in a variety of cells and organisms. This chapter… read more here.
Keywords: crispr cas9; airway biology; biology; human airway ... See more keywords
Human airway trypsin-like protease enhances interleukin-8 synthesis in bronchial epithelial cells by activating protease-activated receptor 2.
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2019.01.019
Abstract: Human airway trypsin-like protease (HAT) localizes at human bronchial epithelial cells (HBECs). HAT enhanced release of interleukin-8 (IL-8) from HBECs at 10-100 mU/mL and the enhanced release was almost completely abolished by 50 μM leupeptin, a… read more here.
Keywords: par2; trypsin like; epithelial cells; protease ... See more keywords
Diesel exhaust particles elevate MUC5AC and MUC5B expression via the TLR4-mediated activation of ERK1/2, p38 MAPK, and NF-κB signaling pathways in human airway epithelial cells.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.02.146
Abstract: Exposure to diesel exhaust particles (DEPs) is known to cause serious health problems, owing to a steady increase in the number of diesel vehicles worldwide. DEPs comprise approximately 90% particle mass existing in the fine… read more here.
Keywords: signaling pathways; epithelial cells; airway epithelial; expression ... See more keywords
Biomass-related PM2.5 induces mitochondrial fragmentation and dysfunction in human airway epithelial cells.
Sign Up to like & getrecommendations! Published in 2021 at "Environmental pollution"
DOI: 10.1016/j.envpol.2021.118464
Abstract: The use of biomass for cooking and heating is considered an important factor associated with chronic obstructive pulmonary disease (COPD), but few studies have previously addressed its underlying mechanisms. Therefore, this research aimed to evaluate… read more here.
Keywords: epithelial cells; dysfunction; airway epithelial; human airway ... See more keywords
Lipid Hydroperoxides as Mediators of Ozone-Induced Oxidative Changes in Human Airway Epithelial Cells
Sign Up to like & getrecommendations! Published in 2017 at "Free Radical Biology and Medicine"
DOI: 10.1016/j.freeradbiomed.2017.10.054
Abstract: Human exposure to tropospheric ozone (O3) is a significant public health concern. O3 inhalation induces airway inflammation and pulmonary function decrements, and may also lead to cardiovascular morbidity. The carbon-carbon double bonds in polyunsaturated fatty… read more here.
Keywords: epithelial cells; oxidative changes; induced oxidative; airway epithelial ... See more keywords
Differential regulation of the transcriptomic and secretomic landscape of sensor and effector functions of human airway epithelial cells
Sign Up to like & getrecommendations! Published in 2018 at "Mucosal Immunology"
DOI: 10.1038/mi.2017.100
Abstract: Protein secretion upon TLR, TNFR1, and IFNGR ligation in the human airways is considered to be central for the orchestration of pulmonary inflammatory and immune responses. In this study, we compared the gene expression and… read more here.
Keywords: epithelial cells; airway epithelial; human airway; effector ... See more keywords
Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17796-z
Abstract: SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in… read more here.
Keywords: hae cultures; morphogenesis cytopathic; airway epithelial; morphogenesis ... See more keywords
Adaptation of influenza A (H7N9) virus in primary human airway epithelial cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-10749-5
Abstract: Influenza A (H7N9) is an emerging zoonotic pathogen with pandemic potential. To understand its adaptation capability, we examined the genetic changes and cellular responses following serial infections of A (H7N9) in primary human airway epithelial… read more here.
Keywords: primary human; h7n9 virus; human airway; influenza h7n9 ... See more keywords
Widespread Sexual Dimorphism in the Transcriptome of Human Airway Epithelium in Response to Smoking
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-54051-y
Abstract: Epidemiological studies have shown that female smokers are at higher risk of chronic obstructive pulmonary disease (COPD). Female patients have worse symptoms and health status and increased risk of exacerbations. We determined the differences in… read more here.
Keywords: response; response smoking; female smokers; human airway ... See more keywords
β-Elemene inhibits the proliferation of primary human airway granulation fibroblasts by down-regulating canonical Wnt/β-catenin pathway
Sign Up to like & getrecommendations! Published in 2018 at "Bioscience Reports"
DOI: 10.1042/bsr20171386
Abstract: Benign airway stenosis is a clinical challenge because of recurrent granulation tissues. Our previous study proved that a Chinese drug, β-elemene, could effectively inhibit the growth of fibroblasts cultured from hyperplastic human airway granulation tissues,… read more here.
Keywords: airway granulation; catenin; canonical wnt; human airway ... See more keywords
48 Understanding the Effects of Quartz Aerosols on Human Airway 3D Models Combined with Primary Macrophages
Sign Up to like & getrecommendations! Published in 2023 at "Annals of Work Exposures and Health"
DOI: 10.1093/annweh/wxac087.124
Abstract: Toxicology assessment using human lung tissue models is critical and complementary for determining the hazards for aerosolized materials. In the present study, we investigated the functional, morphological and reactivity features of reconstituted human airway epithelium… read more here.
Keywords: asthmatic tissues; effects quartz; understanding effects; quartz aerosols ... See more keywords