S100B + A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.25682
Abstract: During monolayer expansion, a necessary step in autologous chondrocyte implantation, human articular chondrocytes (HAC) dedifferentiate and lose their capacity to produce stable hyaline cartilage. Determining HAC potency and learning how to trigger their redifferentiation would… read more here.
Keywords: potency; monolayer; redifferentiation; s100b ... See more keywords
Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase‐1 in human articular chondrocytes
Sign Up to like & getrecommendations! Published in 2018 at "European Journal of Pharmacology"
DOI: 10.1016/j.ejphar.2018.04.018
Abstract: Abstract Osteoarthritis (OA) is common age‐associated disease, and associated with joint pain, mobility limitations and compromised overall quality of life. OA treatment is currently limited to pain management and joint arthroplasty at end stage disease.… read more here.
Keywords: cartilage degeneration; heme oxygenase; articular chondrocytes; human articular ... See more keywords
Wnt5a induces catabolic signaling and matrix metalloproteinase production in human articular chondrocytes.
Sign Up to like & getrecommendations! Published in 2017 at "Osteoarthritis and cartilage"
DOI: 10.1016/j.joca.2017.05.018
Abstract: OBJECTIVE Aberrant Wnt signaling may contribute to osteoarthritis (OA) but the Wnt family members involved have not been fully identified. The purpose of this study was to investigate the role of Wnt5a as a potential… read more here.
Keywords: production; kinase; normal human; wnt5a ... See more keywords
Human Articular Cartilage Mechanosensitivity is Related to Histological Degeneration - A Functional MRI Study.
Sign Up to like & getrecommendations! Published in 2019 at "Osteoarthritis and cartilage"
DOI: 10.1016/j.joca.2019.07.006
Abstract: OBJECTIVE To investigate changes in response to sequential pressure-controlled loading and unloading in human articular cartilage of variable histological degeneration using serial T1ρ mapping. METHOD We obtained 42 cartilage samples of variable degeneration from the… read more here.
Keywords: mri; human articular; degeneration; cartilage ... See more keywords
Complement component C1q is produced by isolated articular chondrocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Osteoarthritis and cartilage"
DOI: 10.1016/j.joca.2019.09.007
Abstract: OBJECTIVE Inflammation and innate immune responses may contribute to development and progression of Osteoarthritis (OA). Chondrocytes are the sole cell type of the articular cartilage and produce extracellular-matrix molecules. How inflammatory mediators reach chondrocytes is… read more here.
Keywords: c1q; articular chondrocytes; mrna expression; c1qa c1qb ... See more keywords
Thickness-wise growth technique for human articular chondrocytes to fabricate three-dimensional cartilage grafts
Sign Up to like & getrecommendations! Published in 2020 at "Regenerative Therapy"
DOI: 10.1016/j.reth.2019.12.001
Abstract: Introduction Cutting the cost of manufacturing is important for extending the use of tissue-engineered therapeutic products. The present study aimed to develop a simple method for fabrication of cartilaginous tissues for regenerative therapy, utilizing the… read more here.
Keywords: wise growth; thickness wise; articular chondrocytes; growth ... See more keywords
Anti-inflammatory and chondroprotective effects of the S-adenosylhomocysteine hydrolase inhibitor 3-Deazaneplanocin A, in human articular chondrocytes
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-06913-6
Abstract: Abstract3-Deazaneplanocin A (DZNep) is an inhibitor of S-Adenosyl-L-Homocysteine Hydrolase (SAHH) known to inhibit EZH2, a histone methylase upregulated during osteoarthritis. In this study, we assessed its effects in human articular chondrocytes. Anti-inflammatory effects were assessed… read more here.
Keywords: anti inflammatory; inhibitor; deazaneplanocin; dznep ... See more keywords
Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential
Sign Up to like & getrecommendations! Published in 2018 at "Cartilage"
DOI: 10.1177/1947603518769714
Abstract: Objective To assess the phenotype of human articular chondrocytes cultured in normoxia (21% O2) or continuous hypoxia (2% O2). Design Chondrocytes were extracted from patients undergoing total knee replacement (n = 5) and cultured in… read more here.
Keywords: phenotype; normoxia promotes; immunomodulatory potential; chondrogenic potency ... See more keywords
Effect of Inflammatory Signaling on Human Articular Chondrocyte Hypertrophy: Potential Involvement of Tissue Repair Macrophages.
Sign Up to like & getrecommendations! Published in 2021 at "Cartilage"
DOI: 10.1177/19476035211021907
Abstract: OBJECTIVE In osteoarthritis, chondrocytes tend to acquire a hypertrophic phenotype, which contributes to the modification of the extracellular matrix, resulting in permanent cartilage changes. In mouse chondrocytes, pro-inflammatory macrophages and pro-inflammatory cytokines have been shown… read more here.
Keywords: tissue repair; inflammatory; inflammatory signaling; hypertrophy ... See more keywords
Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.1046127
Abstract: The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the… read more here.
Keywords: chondrocyte; chondrocyte isolation; human articular; isolation ... See more keywords
Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms22073693
Abstract: Cartilage is a non-innervated and non-vascularized tissue. It is composed of one main cell type, the chondrocyte, which governs homeostasis within the cartilage tissue, but has low metabolic activity. Articular cartilage undergoes substantial stresses that… read more here.
Keywords: collagen; promerim hydrolysates; human articular; synthesis ... See more keywords