Dynamics and function of ERM proteins during cytokinesis in human cells
Sign Up to like & getrecommendations! Published in 2017 at "FEBS Letters"
DOI: 10.1002/1873-3468.12844
Abstract: The molecular mechanism that governs cytoskeleton–membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited… read more here.
Keywords: erm proteins; function erm; human cells; dynamics function ... See more keywords
Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells
Sign Up to like & getrecommendations! Published in 2021 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202102472
Abstract: Safe and efficient production of chimeric antigen receptor (CAR)‐T cells is of crucial importance for cell‐based cancer immunotherapy. Physical transfection methods have quickly gained in importance in the context of transfecting T‐cells, since they are… read more here.
Keywords: polydopamine; human cells; safe efficient; photoporation biodegradable ... See more keywords
Mechanically Tunable Bioink for 3D Bioprinting of Human Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Advanced healthcare materials"
DOI: 10.1002/adhm.201700255
Abstract: This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree of carboxylation, the elastic modulus of printed gels… read more here.
Keywords: bioink; mechanically tunable; bioprinting human; bioink bioprinting ... See more keywords
In‐Cell NMR Spectroscopy of Nucleic Acids in Human Cells
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Nucleic Acid Chemistry"
DOI: 10.1002/cpnc.71
Abstract: In‐cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near‐to‐atomic resolution. In this… read more here.
Keywords: spectroscopy nucleic; nmr spectroscopy; cell nmr; spectroscopy ... See more keywords
Peripheral differentiation patterns of human T cells.
Sign Up to like & getrecommendations! Published in 2022 at "European journal of immunology"
DOI: 10.1002/eji.202149465
Abstract: Long-term T cell memory is dependent on the maintenance of memory T cells in the lymphoid tissues, and at the surface interfaces which provide entry routes for pathogens. However, much of the current information on… read more here.
Keywords: human cells; patterns human; memory; memory subsets ... See more keywords
An optimized platform for efficient siRNA delivery into human NK cells
Sign Up to like & getrecommendations! Published in 2022 at "European Journal of Immunology"
DOI: 10.1002/eji.202149710
Abstract: The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents a… read more here.
Keywords: human cells; efficient sirna; optimized platform; platform efficient ... See more keywords
Assessing gene function in human B cells: CRISPR/Cas9‐based gene editing and mRNA‐based gene expression in healthy and tumor cells
Sign Up to like & getrecommendations! Published in 2022 at "European Journal of Immunology"
DOI: 10.1002/eji.202149784
Abstract: Robust protocols for genetic manipulation are highly needed to facilitate study of gene function in human B cells, both in settings of health and disease. B cells are notoriously hard to manipulate, being especially fragile… read more here.
Keywords: based gene; function human; human cells; gene function ... See more keywords
Direct RIG‐I activation in human NK cells induces TRAIL‐dependent cytotoxicity toward autologous melanoma cells
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Cancer"
DOI: 10.1002/ijc.31874
Abstract: Activation of the innate immune receptor retinoic acid‐inducible gene I (RIG‐I) by its specific ligand 5′‐triphosphate RNA (3pRNA) triggers anti‐tumor immunity, which is dependent on natural killer (NK) cell activation and cytokine induction. However, to… read more here.
Keywords: rig activation; rig; melanoma cells; activation ... See more keywords
Reviving human γδT cells from apoptosis induced by IL‐12/18 via p‐JNK inhibition
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Leukocyte Biology"
DOI: 10.1002/jlb.5ma0622-741r
Abstract: γδT cells recognize and exert cytotoxicity against tumor cells independently of MHC restriction and have antigen presentation and regulatory functions to promote adaptive immune responses. They are considered as potential immune cells for cellular immunotherapy… read more here.
Keywords: reviving human; apoptosis; human cells; cells apoptosis ... See more keywords
Selectable high‐yield recombinant protein production in human cells using a GFP/YFP nanobody affinity support
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3409
Abstract: Recombinant protein expression systems that produce high yields of pure proteins and multi‐protein complexes are essential to meet the needs of biologists, biochemists, and structural biologists using X‐ray crystallography and cryo‐electron microscopy. An ideal expression… read more here.
Keywords: protein; yfp nanobody; gfp yfp; expression ... See more keywords
Doxorubicin induces ZAKα overexpression with a subsequent enhancement of apoptosis and attenuation of survivability in human osteosarcoma cells
Sign Up to like & getrecommendations! Published in 2018 at "Environmental Toxicology"
DOI: 10.1002/tox.22507
Abstract: Human osteosarcoma (OS) is a malignant cancer of the bone. It exhibits a characteristic malignant osteoblastic transformation and produces a diseased osteoid. A previous study demonstrated that doxorubicin (DOX) chemotherapy decreases human OS cell proliferation… read more here.
Keywords: apoptosis; effect; human osteosarcoma; zak overexpression ... See more keywords