Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in cancer therapy
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28312
Abstract: Nanoparticles are used as carriers for the delivery of drugs and imaging agents. Proteins are safer than synthetic nanocarriers due to their greater biocompatibility and the absence of toxic degradation products. In this context, ferritin… read more here.
Keywords: human ferritin; heavy chain; production; delivery ... See more keywords
Electroinduced Extraction of Human Ferritin Heavy Chain Expressed in Hansenula polymorpha
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2627-9
Abstract: AbstractА protocol for the efficient and selective recovery of human ferritin heavy chain (FTH1) expressed intracellularly in Hansenula polymorpha was developed. It was based on electropermeabilisation and an increase in the cell wall porosity by… read more here.
Keywords: ferritin heavy; heavy chain; human ferritin; polymorpha ... See more keywords
Development of robust and facile purification process for production of recombinant human ferritin heavy chain nanoparticle from Escherichia coli
Sign Up to like & getrecommendations! Published in 2021 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2021.02.014
Abstract: Abstract Recombinant human ferritin heavy chain (rhFTH) was highly expressed and self-assembled in E. coli bacteria. To establish a robust purification scheme, we developed a non-chromatographic precipitation procedure as a key step for extracting rhFTH… read more here.
Keywords: human ferritin; ferritin heavy; recombinant human; heavy chain ... See more keywords