Optogenetic Modulation and Reprogramming of Bacteriorhodopsin‐Transfected Human Fibroblasts on Self‐Assembled Fullerene C60 Nanosheets
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Biosystems"
DOI: 10.1002/adbi.201800254
Abstract: Fullerenes have unique biocompatibility and photoelectric properties and are candidate materials for biomedical applications. Several cell membrane proteins in nature such as bacteriorhodopsin also have photoelectric properties. Highly expressible bacteriorhodopsin (HEBR) is a novel light‐sensitive… read more here.
Keywords: optogenetic modulation; human fibroblasts; self assembled; photoelectric properties ... See more keywords
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1084-8_7
Abstract: Much investigation is needed to understand the underlying molecular mechanisms of iPSC reprogramming and to improve this technology. Lentivirus-mediated iPSC reprogramming remains the most effective method to study human pluripotency reprogramming. iPSC production is more… read more here.
Keywords: using gfp; defined medium; human fibroblasts; lentiviral vectors ... See more keywords
Novel resveratrol derivatives have diverse effects on the survival, proliferation and senescence of primary human fibroblasts
Sign Up to like & getrecommendations! Published in 2020 at "Biogerontology"
DOI: 10.1007/s10522-020-09896-6
Abstract: Resveratrol alters the cytokinetics of mammalian cell populations in a dose dependent manner. Concentrations above 25–50 µM typically trigger growth arrest, senescence and/or apoptosis in multiple different cell types. In contrast, concentrations below 10 µM… read more here.
Keywords: resveratrol; senescence primary; senescence; human fibroblasts ... See more keywords
The pleiotropic effects of Prunus avium L. extract against oxidative stress on human fibroblasts. An in vitro approach.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-021-06464-0
Abstract: There is a persistent interest in innovative and multifunctional ingredients in biology research. With regards to this, natural sources have an important role due to their multiple benefits. Thus, this study aims to present the… read more here.
Keywords: prunus avium; human fibroblasts; avium extract; biology ... See more keywords
Reciprocal regulation of acetyl-CoA carboxylase 1 and senescence in human fibroblasts involves oxidant mediated p38 MAPK activation.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2016.10.016
Abstract: We sought to explore the fate of the fatty acid synthesis pathway in human fibroblasts exposed to DNA damaging agents capable of inducing senescence, a state of irreversible growth arrest. Induction of premature senescence by… read more here.
Keywords: coa carboxylase; fatty acid; senescence; human fibroblasts ... See more keywords
OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts.
Sign Up to like & getrecommendations! Published in 2017 at "Cell reports"
DOI: 10.1016/j.celrep.2017.07.071
Abstract: SOX2 and OCT4, in conjunction with KLF4 and cMYC, are sufficient to reprogram human fibroblasts to induced pluripotent stem cells (iPSCs), but it is unclear if they function as transcriptional activators or as repressors. We… read more here.
Keywords: sox2 work; human fibroblasts; sox2 vp16; oct4 sox2 ... See more keywords
Simultaneous reprogramming and gene editing of human fibroblasts.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2018.007
Abstract: The utility of human induced pluripotent stem cells (iPSCs) is enhanced by an ability to precisely modify a chosen locus with minimal impact on the remaining genome. However, the derivation of gene-edited iPSCs typically involves… read more here.
Keywords: gene editing; human fibroblasts; gene edited; simultaneous reprogramming ... See more keywords
Self-assembled Collagen-Fibrin Hydrogel Reinforces Tissue Engineered Adventitia Vessels Seeded with Human Fibroblasts
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-21681-7
Abstract: Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel,… read more here.
Keywords: collagen; strength; human fibroblasts; tissue ... See more keywords
Direct phenotypic conversion of human fibroblasts into functional osteoblasts triggered by a blockade of the transforming growth factor-β signal
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26745-2
Abstract: A procedure to generate functional osteoblasts from human somatic cells may pave the way to a novel and effective transplantation therapy in bone disorders. Here, we report that human fibroblasts were induced to show osteoblast… read more here.
Keywords: human fibroblasts; growth factor; bone; functional osteoblasts ... See more keywords
Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-52294-3
Abstract: Oct4-mediated reprogramming has recently become a novel tool for the generation of various cell types from differentiated somatic cells. Although molecular mechanisms underlying this process are unknown, it is well documented that cells over-expressing Oct4… read more here.
Keywords: state; microrna expression; oct4 mediated; human fibroblasts ... See more keywords
Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep40285
Abstract: Transdifferentiation has been described as a novel method for converting human fibroblasts into induced cardiomyocyte-like cells. Such an approach can produce differentiated cells to study physiology or pathophysiology, examine drug interactions or toxicities, and engineer… read more here.
Keywords: transcription; transdifferentiation; human fibroblasts; transdifferentiation human ... See more keywords