Engraftment of Human Hematopoietic Cells in Biomaterials Implanted in Immunodeficient Mouse Models.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1425-9_18
Abstract: Over the last 20 years, significant progress has been made in the development of immunodeficient mouse models that now represents the gold standard tool in stem cell biology research. The latest major improvement has been the… read more here.
Keywords: mouse models; immunodeficient mouse; engraftment human; human hematopoietic ... See more keywords
In vitro large scale production of megakaryocytes to functional platelets from human hematopoietic stem cells.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.09.090
Abstract: Megakaryocytopoiesis results in the formation of platelets, which are essential for hemostasis. Decreased production or increased destruction of platelets can cause thrombocytopenia, in which platelet transfusion is the mode of treatment. The present study is… read more here.
Keywords: hscs; production; human hematopoietic; hematopoietic stem ... See more keywords
Senescent human hematopoietic progenitors show elevated expression of transposable elements and inflammatory genes.
Sign Up to like & getrecommendations! Published in 2018 at "Experimental hematology"
DOI: 10.1016/j.exphem.2018.03.003
Abstract: Genomic transposable elements (TEs) constitute the majority of the genome. Expression of TEs is known to activate the double-stranded RNA recognition pathway ("viral mimicry"), leading to the activation of interferon-stimulated genes, inflammation, and immune-mediated cell… read more here.
Keywords: expression tes; senescence; human hematopoietic; inflammatory genes ... See more keywords
ITERATIVE AND PLEIOTROPIC ROLES OF ENDOTHELIAL CELLS IN HUMAN HEMATOPOIETIC ERYTHROPOIESIS
Sign Up to like & getrecommendations! Published in 2019 at "Experimental Hematology"
DOI: 10.1016/j.exphem.2019.06.442
Abstract: Local tissue microenvironments regulate directly the hematopoietic development and cell fate specification of both stem cells and lineage-committed progenitor cells. The identification of hematopoietic niches and the mechanisms by which they regulate cell fate specification… read more here.
Keywords: erythropoiesis; human hematopoietic; iterative pleiotropic; endothelial cells ... See more keywords
CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation
Sign Up to like & getrecommendations! Published in 2019 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2019.04.023
Abstract: Summary Hematopoietic stem and progenitor cells (HSPCs) depend on regulatory cytokines from the marrow microenvironment. From an unbiased cytokine screen of murine marrow supernatants, we identified C-C motif chemokine ligand 5 (CCL5) as an endothelial… read more here.
Keywords: hematopoietic regeneration; regeneration; human hematopoietic; ccr5 ... See more keywords
Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abm9106
Abstract: While CRISPR-Cas9 is key for the development of gene therapy, its potential off-target mutations are still a major concern. Here, we establish a “spacer-nick” gene correction approach that combines the Cas9D10A nickase with a pair… read more here.
Keywords: target mutations; human hematopoietic; hematopoietic stem; spacer nick ... See more keywords
Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia.
Sign Up to like & getrecommendations! Published in 2019 at "Cancer discovery"
DOI: 10.1158/2159-8290.cd-18-1474
Abstract: Aging is associated with functional decline of hematopoietic stem cells (HSC) as well as an increased risk of myeloid malignancies. We performed an integrative characterization of epigenomic and transcriptomic changes, including single-cell RNA-seq, during normal… read more here.
Keywords: epigenetic reprogramming; aging human; stem cells; hematopoietic stem ... See more keywords
Eltrombopag maintains human hematopoietic stem and progenitor cells under inflammatory conditions mediated by IFN-γ.
Sign Up to like & getrecommendations! Published in 2019 at "Blood"
DOI: 10.1182/blood-2018-11-884486
Abstract: The proinflammatory cytokine interferon-γ (IFN-γ) has been implicated in human hematopoietic stem and progenitor cell (HSPC) depletion in immune-mediated bone marrow failure syndromes. We show that IFN-γ specifically prevents full engagement of thrombopoietin (TPO), a… read more here.
Keywords: tpo; stem progenitor; human hematopoietic; hematopoietic stem ... See more keywords
HLF Expression Defines the Human Hematopoietic Stem Cell State.
Sign Up to like & getrecommendations! Published in 2021 at "Blood"
DOI: 10.1182/blood.2021010745
Abstract: Hematopoietic stem cells (HSCs) sustain blood cell homeostasis throughout life and can regenerate all blood lineages following transplantation. Despite this clear functional definition, highly enriched isolation of human HSCs can currently only be achieved through… read more here.
Keywords: human hematopoietic; stem cell; blood; hematopoietic stem ... See more keywords
Aging alters the cell cycle control and mitogenic signaling responses of human hematopoietic stem cells.
Sign Up to like & getrecommendations! Published in 2023 at "Blood"
DOI: 10.1182/blood.2022017174
Abstract: Human hematopoietic stem cells (HSCs), like their counterparts in mice, comprise a functionally and molecularly heterogeneous population of cells throughout life that, collectively, maintain required outputs of mature blood cellsunder homeostatic conditions. An early developmental… read more here.
Keywords: human hematopoietic; cell cycle; hematopoietic stem; blood ... See more keywords
Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44.
Sign Up to like & getrecommendations! Published in 2017 at "Blood advances"
DOI: 10.1182/bloodadvances.2017004317
Abstract: CD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their… read more here.
Keywords: cd34; progenitor cells; human hematopoietic; stem progenitor ... See more keywords