Decellularized Liver Nanofibers Enhance and Stabilize the Long-term Functions of Primary Human Hepatocytes In Vitro.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202202302
Abstract: Owing to significant differences across species in liver functions, in vitro human liver models are used for screening the metabolism and toxicity of compounds, modeling diseases, and cell-based therapies. However, the extracellular matrix (ECM) scaffold… read more here.
Keywords: human hepatocytes; liver; primary human; topography ... See more keywords
Transcriptomic Analysis of Cholestatic Compounds In Vitro.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_12
Abstract: Drug-induced cholestasis is one of the most severe manifestations of drug-induced liver injury. Drug-induced cholestasis is characterized by an accumulation of endogenous metabolites normally excreted in the bile such as bile salts, cholesterol, bilirubin, or… read more here.
Keywords: drug induced; drug; induced cholestasis; primary human ... See more keywords
Establishment of Sandwich Cultures of Primary Human Hepatocytes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9420-5_21
Abstract: Primary hepatocytes and their adherent cultures are still considered as the golden standard in the field of liver-based in vitro modeling. However, they cope with progressive deterioration of their in vivo-like morphological and functional phenotype.… read more here.
Keywords: cultures primary; establishment sandwich; biology; sandwich cultures ... See more keywords
Assessment of long-term functional maintenance of primary human hepatocytes to predict drug-induced hepatoxicity in vitro.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03050-y
Abstract: Hepatocytes are the main cell components of the liver and perform metabolic, detoxification, and endocrine functions. Functional hepatocytes are of great value in drug development, toxicity evaluation, and cell therapy for liver diseases. In recent years,… read more here.
Keywords: long term; drug; vitro; primary human ... See more keywords
Gene expression profiling of human hepatocytes grown on differing substrate stiffness
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-2536-1
Abstract: ObjectiveTo study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.ResultsWith the aid of RNA-Seq technology, our study… read more here.
Keywords: stiffness; substrate stiffness; pathology; rna seq ... See more keywords
Metabolism of the new synthetic cannabinoid EG-018 in human hepatocytes by high-resolution mass spectrometry
Sign Up to like & getrecommendations! Published in 2018 at "Forensic Toxicology"
DOI: 10.1007/s11419-018-0404-2
Abstract: PurposeThe present study aims to recommend appropriate urinary marker metabolites for documenting EG-018 consumption by investigating its metabolism in human hepatocytes.MethodsFor metabolite profiling, 10 µM EG-018 was incubated in human hepatocytes for 3 h. Metabolite identification in… read more here.
Keywords: high resolution; 018 human; mass spectrometry; metabolism ... See more keywords
Application of MetMax™ pooled donor human hepatocytes in a higher throughput assay for human hepatic metabolic stability screening
Sign Up to like & getrecommendations! Published in 2018 at "Drug Metabolism and Pharmacokinetics"
DOI: 10.1016/j.dmpk.2017.11.208
Abstract: • Both MetMaxTM and Intact Human Hepatocytes yielded data comparable to clinical systemic clearance One interesting finding was that MetMaxTM hepatocytes were superior to pooled cryopreserved human hepatocytes in the evaluation of afatinib, a drug… read more here.
Keywords: stability screening; metabolic stability; pooled donor; drug ... See more keywords
Derivation of CYP3A4 and CYP2B6 degradation rate constants in primary human hepatocytes: A siRNA-silencing-based approach.
Sign Up to like & getrecommendations! Published in 2018 at "Drug metabolism and pharmacokinetics"
DOI: 10.1016/j.dmpk.2018.01.004
Abstract: The first-order degradation rate constant (kdeg) of cytochrome P450 (CYP) enzymes is a known source of uncertainty in the prediction of time-dependent drug-drug interactions (DDIs) in physiologically-based pharmacokinetic (PBPK) modelling. This study aimed to measure… read more here.
Keywords: degradation rate; cyp3a4 cyp2b6; cyp3a4; primary human ... See more keywords
Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and Cellular Endocrinology"
DOI: 10.1016/j.mce.2019.110604
Abstract: Until three decades, ago 3,5-diiodothyronine (3,5-T2) and 3,3'-diiodothyronine (3,3'-T2) were considered products of thyroid hormone catabolism without biological activity. Some metabolic effects have been described in rodents, but the physiological relevance in humans and the… read more here.
Keywords: diiodothyronines regulate; metabolic homeostasis; human hepatocytes; primary human ... See more keywords
Replacement per- and polyfluoroalkyl substances (PFAS) are potent modulators of lipogenic and drug metabolizing gene expression signatures in primary human hepatocytes.
Sign Up to like & getrecommendations! Published in 2022 at "Toxicology and applied pharmacology"
DOI: 10.1016/j.taap.2022.115991
Abstract: Per- and polyfluoroalkyl substances (PFAS) are a class of environmental toxicants, and some, such as perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), have been associated with hepatic steatosis in rodents and monkeys. It was hypothesized… read more here.
Keywords: human hepatocytes; substances pfas; per polyfluoroalkyl; gene ... See more keywords
Functional Analysis of Human Hepatocytes Isolated From Chimeric Mouse Liver.
Sign Up to like & getrecommendations! Published in 2018 at "Transplantation proceedings"
DOI: 10.1016/j.transproceed.2018.06.035
Abstract: Chimeric mice with humanized liver were first established by transplanting primary human hepatocytes (PHHs) isolated from a Japanese 27-year-old donor into complementary DNA-urokinase-type plasminogen activator/severe combined immunodeficiency mice. The PHHs from the Japanese donor increased… read more here.
Keywords: analysis human; chimeric mouse; human hepatocytes; mouse liver ... See more keywords