Evaluation of anti-insulin receptor antibodies as potential novel therapies for human insulin receptoropathy using cell culture models
Sign Up to like & getrecommendations! Published in 2018 at "Diabetologia"
DOI: 10.1007/s00125-018-4606-2
Abstract: Aims/hypothesisBi-allelic loss-of-function mutations in the INSR gene (encoding the insulin receptor [INSR]) commonly cause extreme insulin resistance and early mortality. Therapeutic options are limited, but anti-INSR antibodies have been shown to activate two mutant receptors,… read more here.
Keywords: insulin; insulin receptor; insr; insulin receptoropathy ... See more keywords
Origins of the Crisis in Insulin Affordability and Practical Advice for Clinicians on Using Human Insulin
Sign Up to like & getrecommendations! Published in 2020 at "Current Diabetes Reports"
DOI: 10.1007/s11892-020-1286-3
Abstract: Purpose of Review High insulin prices and cost-related insulin underuse are increasingly common and vexing problems for healthcare providers. This review highlights several factors that contribute to high prices and limited generic competition in the… read more here.
Keywords: insulin; origins crisis; insulin affordability; crisis insulin ... See more keywords
Polysorbate 80 controls Morphology, structure and stability of human insulin Amyloid-Like spherulites.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of colloid and interface science"
DOI: 10.1016/j.jcis.2021.09.132
Abstract: Amyloid protein aggregates are not only associated with neurodegenerative diseases and may also occur as unwanted by-products in protein-based therapeutics. Surfactants are often employed to stabilize protein formulations and reduce the risk of aggregation. However,… read more here.
Keywords: insulin; aggregation; morphology; insulin amyloid ... See more keywords
Utilizing ELISA‐plate based immunopurification and liquid chromatography‐tandem mass spectrometry for the urinary detection of short‐ and long acting human insulin analogues
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.02.024
Abstract: HighlightsQualitative measurement of human urinary insulin analogues is described.Ultrafiltration and immunoaffinity purification are utilized.LODs in the low pg/ml range are achieved using conventional HPLC system.Proof of principle is shown by the analysis of excretion samples.… read more here.
Keywords: insulin; detection; insulin analogues; liquid chromatography ... See more keywords
Comparative analysis of buffalo (Bubalus bubalis) non-transgenic and transgenic embryos containing human insulin gene, produced by SCNT.
Sign Up to like & getrecommendations! Published in 2019 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2019.06.004
Abstract: Somatic cell nuclear transfer (SCNT), using transgenic donor cells, is a highly efficient method for producing transgenic embryos. We compared the developmental competence, quality and gene expression of transgenic embryos produced by Hand-made cloning from… read more here.
Keywords: embryos; non transgenic; transgenic embryos; gene ... See more keywords
Molecular Determinants of Substrate Specificity in Human Insulin-Degrading Enzyme.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00474
Abstract: Insulin-degrading enzyme (IDE) is a 110 kDa chambered zinc metalloendopeptidase that degrades insulin, amyloid β, and other intermediate-sized aggregation prone peptides that adopt β-structures. Structural studies of IDE in complex with multiple physiological substrates have… read more here.
Keywords: insulin; degrading enzyme; insulin degrading; active site ... See more keywords
Effect of Differences in the Primary Structure of the A-Chain on the Aggregation of Insulin Fragments
Sign Up to like & getrecommendations! Published in 2018 at "ACS Omega"
DOI: 10.1021/acsomega.8b00500
Abstract: Bovine and human insulin have similar primary structures. In this article, the region of the insulin A-chain of bovine and human insulin where the amino acid composition is different was studied. Bovine insulin fragment (BIF)… read more here.
Keywords: insulin; phase; aggregation; chain ... See more keywords
Adsorption and immobilisation of human insulin on graphene monoxide, silicon carbide and boron nitride nanosheets investigated by molecular dynamics simulation
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Simulation"
DOI: 10.1080/08927022.2016.1270452
Abstract: Abstract The adsorption and immobilisation of human insulin onto the bio-compatible nanosheets including graphene monoxide, silicon carbide and boron nitride nanosheets were studied by molecular dynamics simulation at the temperature of 310 K. After equilibration,… read more here.
Keywords: insulin; adsorption; human insulin; simulation ... See more keywords
A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli)
Sign Up to like & getrecommendations! Published in 2020 at "AMB Express"
DOI: 10.1186/s13568-020-00969-w
Abstract: Insulin has captured researchers’ attention worldwide. There is a rapid global rise in the number of diabetic patients, which increases the demand for insulin. Current methods of insulin production are expensive and time-consuming. A PCR-based… read more here.
Keywords: novel efficient; insulin; protein; insulin production ... See more keywords
SAT-171 Effective Intrajejunal Wall Delivery of Recombinant Human Insulin via a Self-Deployed Ingestible Capsule in Anesthetized Swines: A Pharmacokinetic and Pharmacodynamic Study
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the Endocrine Society"
DOI: 10.1210/js.2019-sat-171
Abstract: Abstract BACKGROUND: Biotherapeutic agents must be administered parenterally to reach and maintain therapeutic blood concentrations, lowering patient compliance and complicating long-term care. We have developed an ingestible robotic pill capable of delivering a variety of… read more here.
Keywords: recombinant human; insulin; jejunal wall; human insulin ... See more keywords
Effect of recombinant human insulin-like growth factor 1 therapy in a child with 3-M syndrome-1 with CUL7 gene mutation
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Pediatric Endocrinology and Metabolism"
DOI: 10.1515/jpem-2020-0278
Abstract: Abstract Objective 3-M syndrome is characterized by severe short stature, syndromic features, and characteristic radiographic findings. Growth hormone (GH) has been used with variable success. Recombinant human insulin like growth factor-1 (rhIGF-1) has never been… read more here.
Keywords: recombinant human; human insulin; child; therapy ... See more keywords