Induction of Functional Hepatocytes from Human iPSCs.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8961-4_12
Abstract: Human-induced pluripotent stem cells (iPSCs) could be a useful source for production of hepatocytes. Here, we develop protocols to generate iPSC-derived liver progenitor cells, liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs). We… read more here.
Keywords: hepatocytes human; functional hepatocytes; induction functional; biology ... See more keywords
Serotonin-specific neurons differentiated from human iPSCs form distinct subtypes with synaptic protein assembly
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Neural Transmission"
DOI: 10.1007/s00702-021-02303-5
Abstract: Human induced pluripotent stem cells (hiPSCs) have revolutionized the generation of experimental disease models, but the development of protocols for the differentiation of functionally active neuronal subtypes with defined specification is still in its infancy.… read more here.
Keywords: serotonin specific; differentiated human; neurons differentiated; specific neurons ... See more keywords
Carboxylated nanodiamond-mediated CRISPR-Cas9 delivery of human retinoschisis mutation into human iPSCs and mouse retina.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2019.10.037
Abstract: Nanodiamonds (NDs) are considered to be relatively safe carbon nanomaterials used for the transmission of DNA, proteins and drugs. The feasibility of utilizing the NDs to deliver CRISPR-Cas9 system for gene editing has not been… read more here.
Keywords: crispr cas9; ipscs mouse; human ipscs; cas9 ... See more keywords
One-step induction of photoreceptor-like cells from human iPSCs by delivering transcription factors
Sign Up to like & getrecommendations! Published in 2022 at "iScience"
DOI: 10.1016/j.isci.2022.103987
Abstract: Summary Retinal dystrophies (RDs) lead to irreversible vision impairment with no radical treatment. Although photoreceptor cells (PRCs) differentiated from human induced pluripotent stem cells (iPSCs) are essential for the study of RDs as a scalable… read more here.
Keywords: like cells; photoreceptor like; transcription factors; human ipscs ... See more keywords
Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools.
Sign Up to like & getrecommendations! Published in 2021 at "Methods"
DOI: 10.1016/j.ymeth.2021.01.002
Abstract: Mutagenesis studies have rapidly evolved in the era of CRISPR genome editing. Precise manipulation of genes in human induced pluripotent stem cells (iPSCs) allows biomedical researchers to study the physiological functions of individual genes during… read more here.
Keywords: genome editing; human ipscs; targeted mutagenesis; crispr genome ... See more keywords
Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-89312-2
Abstract: Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines. However, in typical protocols, mutations are intentionally introduced into the donor template to avoid the cleavage of donor template or re-cleavage of the successfully… read more here.
Keywords: guided cas9; genome editing; single guided; human ipscs ... See more keywords
HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells
Sign Up to like & getrecommendations! Published in 2021 at "Stem Cells International"
DOI: 10.1155/2021/8873383
Abstract: Although human induced pluripotent stem cells (iPSCs) can serve as a universal cell source for regenerative medicine, the use of iPSCs in clinical applications is limited by prohibitive costs and prolonged generation time. Moreover, allogeneic… read more here.
Keywords: genome editing; ipscs; hla; dendritic cells ... See more keywords
Convergence of Three-Dimensional Cell Culture and Human iPS Cells: Improving Clinical Relevance in Drug Discovery
Sign Up to like & getrecommendations! Published in 2019 at "SLAS Technology"
DOI: 10.1177/2472630318810819
Abstract: In the last decade, the adoption of advanced three-dimensional (3D) cell culture technologies has accelerated in basic research, drug discovery, and development. This has occurred in parallel with the growing use of human cells derived… read more here.
Keywords: cell culture; human ipscs; drug discovery; culture ... See more keywords
Differentiation of human iPSCs into functional podocytes
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0203869
Abstract: Podocytes play a critical role in glomerular barrier function, both in health and disease. However, in vivo terminally differentiated podocytes are difficult to be maintained in in vitro culture. Induced pluripotent stem cells (iPSCs) offer… read more here.
Keywords: like cells; differentiation human; differentiation; podocyte ... See more keywords
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.83291
Abstract: An in vitro model of human ovarian follicles would greatly benefit the study of female reproduction. Ovarian development requires the combination of germ cells and their supporting somatic cells, known as granulosa cells. Whereas efficient… read more here.
Keywords: human ipscs; granulosa like; overexpression; differentiation human ... See more keywords