An Organotypic 3D Assay for Primary Human Mammary Epithelial Cells that Recapitulates Branching Morphogenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7021-6_9
Abstract: We have developed a three-dimensional organotypic culture system for primary human mammary epithelial cells (HMECs) in which the cells are cultured in free floating collagen type I gels. In this assay, luminal cells predominantly form… read more here.
Keywords: epithelial cells; mammary epithelial; branching morphogenesis; human mammary ... See more keywords
Murine and Human Mammary Cancer Cell Lines: Functional Tests.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8600-2_17
Abstract: The biological characterization of mammary cancer cells is a prerequisite that helps the scientist understand some aspect of tumor biology. Once isolated from the tumor, cells are subjected to multiple tests that dissect their ability… read more here.
Keywords: human mammary; biology; cancer cell; murine human ... See more keywords
Cell culture of the normal human mammary gland cultivated in monolayer - A mini systematic review.
Sign Up to like & getrecommendations! Published in 2021 at "Acta histochemica"
DOI: 10.1016/j.acthis.2021.151798
Abstract: The mammary glands are constituted of different cell types. For example, the epithelial cells appear as the target in many studies since they produce and secrete milk during lactation and are the origin of many… read more here.
Keywords: mammary gland; human mammary; culture; cell culture ... See more keywords
Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17102-x
Abstract: The mammary gland is a highly vascularized tissue capable of expansion and regression during development and disease. To enable mechanistic insight into the coordinated morphogenic crosstalk between the epithelium and vasculature, we introduce a 3D… read more here.
Keywords: dysfunction; mammary duct; human mammary; platform ... See more keywords
The transcriptome of human mammary epithelial cells infected with the HCMV-DB strain displays oncogenic traits
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-30109-1
Abstract: Increasing evidence indicates that human cytomegalovirus (HCMV) populations under the influence of host environment, can either be stable or rapidly differentiating, leading to tissue compartment colonization. We isolated previously from a 30-years old pregnant woman,… read more here.
Keywords: hcmv strain; epithelial cells; mammary epithelial; hcmv ... See more keywords
Bisphenol A replacement chemicals, BPF and BPS, induce protumorigenic changes in human mammary gland organoid morphology and proteome
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2115308119
Abstract: Significance Bisphenol A (BPA), found in many plastic products, has weak estrogenic effects that can be harmful to human health. Thus, structurally related replacements—bisphenol S (BPS) and bisphenol F (BPF)—are coming into wider use with… read more here.
Keywords: human mammary; bps; replacement chemicals; bisphenol replacement ... See more keywords
Citronellol, an Acyclic Monoterpene Induces Mitochondrial-Mediated Apoptosis through Activation of Proapoptotic Factors in MCF-7 and MDA-MB-231 Human Mammary Tumor Cells
Sign Up to like & getrecommendations! Published in 2020 at "Nutrition and Cancer"
DOI: 10.1080/01635581.2020.1800766
Abstract: Abstract The present study investigated the anticancer activity of citronellol (CT) by analyzing the mitochondrial-mediated activation of apoptosis in MCF-7 and MDA-MB-231 human mammary tumor cell lines. Cytotoxicity, cell growth, and apoptosis were determined by… read more here.
Keywords: mda 231; apoptosis; mcf mda; human mammary ... See more keywords
Mechanisms of HERV-K (HML-2) Transcription during Human Mammary Epithelial Cell Transformation
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Virology"
DOI: 10.1128/jvi.01258-17
Abstract: ABSTRACT Increasing evidence suggests that repetitive elements may play a role in host gene regulation, particularly through the donation of alternative promoters, enhancers, splice sites, and termination signals. Elevated transcript expression of the endogenous retrovirus… read more here.
Keywords: transcription; mammary epithelial; epithelial cell; human mammary ... See more keywords
Abstract 6519: Haploinsufficiency for BRCA2 leads to common fragile site instability in human mammary epithelial cells
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-6519
Abstract: Individuals with germline heterozygous mutations of breast cancer susceptibility genes BRCA1 or BRCA2 have a 50 to 80% lifetime risk of developing breast cancer, yet the mechanisms of these cancers remain largely unknown. There is… read more here.
Keywords: human mammary; instability; epithelial cells; mammary epithelial ... See more keywords
Abstract A59: Assessment of conditional reprogramming to generate 2D and 3D primary human mammary cell culture models
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Cancer Research"
DOI: 10.1158/1557-3125.advbc17-a59
Abstract: Human mammary gland development and differentiation are tightly regulated by hormones, growth factors, and microenvironmental cues. Rodent models have been used to help gain knowledge about mammary gland biology, but there are significant structural and… read more here.
Keywords: conditional reprogramming; mammary cell; human mammary; culture ... See more keywords
1,25-Dihydroxyvitamin D Regulation of Glutamine Synthetase and Glutamine Metabolism in Human Mammary Epithelial Cells
Sign Up to like & getrecommendations! Published in 2017 at "Endocrinology"
DOI: 10.1210/en.2017-00238
Abstract: Genomic profiling has identified a subset of metabolic genes that are altered by 1,25-dihydroxyvitamin D (1,25D) in breast cells, including GLUL, the gene that encodes glutamine synthetase (GS). In this study, we explored the relevance… read more here.
Keywords: epithelial cells; mammary epithelial; glutamine synthetase; human mammary ... See more keywords