Bovine natural antibody IgM inhibits the binding of human norovirus protruding domain to its HBGA receptors
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13450
Abstract: Human norovirus (HuNoV) is the primary viral pathogen that causes acute gastroenteritis (AGE) in humans. The protruding (P) domain of HuNoV interacts with cell surface histo‐blood group antigens (HBGAs) to initiate infection. Owing to the… read more here.
Keywords: natural antibody; hbga receptors; protruding domain; domain ... See more keywords
Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9597-4_8
Abstract: The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay… read more here.
Keywords: lips assay; human norovirus; system lips; immunoprecipitation system ... See more keywords
Mori Cortex Radicis extract inhibits human norovirus surrogate in simulated digestive conditions
Sign Up to like & getrecommendations! Published in 2021 at "Food Science and Biotechnology"
DOI: 10.1007/s10068-021-00958-0
Abstract: Norovirus is a major cause of acute gastroenteritis globally, resulting in enormous health and societal costs. In this study, the antiviral activities of Mori Cortex Radicis (MCR) extract and its bioactive flavonoids, morusin and kuwanon… read more here.
Keywords: simulated digestive; mori cortex; human norovirus; norovirus surrogate ... See more keywords
Complete assignment of Ala, Ile, LeuProS, Met and ValProS methyl groups of the protruding domain from human norovirus GII.4 Saga
Sign Up to like & getrecommendations! Published in 2020 at "Biomolecular Nmr Assignments"
DOI: 10.1007/s12104-020-09932-z
Abstract: Attachment of human noroviruses to histo blood group antigens (HBGAs) is thought to be essential for infection, although how this binding event promotes infection is unknown. Recent studies have shown that 60% of all GII.4… read more here.
Keywords: methyl; complete assignment; human norovirus; gii saga ... See more keywords
Development of a Specific Anti-capsid Antibody- and Magnetic Bead-Based Immunoassay to Detect Human Norovirus Particles in Stool Samples and Spiked Mussels via Flow Cytometry.
Sign Up to like & getrecommendations! Published in 2021 at "Food and environmental virology"
DOI: 10.1007/s12560-021-09494-w
Abstract: Human noroviruses impose a considerable health burden globally. Here, a flow cytometry approach designed for their detection in biological waste and food samples was developed using antibody-coated magnetic beads. Antipeptide antibodies against murine norovirus and… read more here.
Keywords: stool samples; detection; human norovirus; flow cytometry ... See more keywords
A plate-based histo-blood group antigen binding assay for evaluation of human norovirus receptor binding ability.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.06.012
Abstract: Human norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid… read more here.
Keywords: plate based; binding assay; receptor binding; human norovirus ... See more keywords
Characterizing the effects of thermal treatment on human norovirus GII.4 viability using propidium monoazide combined with RT-qPCR and quality assessments in mussels
Sign Up to like & getrecommendations! Published in 2020 at "Food Control"
DOI: 10.1016/j.foodcont.2019.106954
Abstract: Abstract This study investigated the effects of thermal treatment (70, 80, and 90 °C for 5, 10, and 20 min) on the titers of human norovirus (HuNoV) GII.4, a dominant genotype in fresh mussels using propidium monoazide… read more here.
Keywords: propidium monoazide; human norovirus; using propidium; effects thermal ... See more keywords
Human Norovirus Propagation in Human Induced Pluripotent Stem Cell–Derived Intestinal Epithelial Cells
Sign Up to like & getrecommendations! Published in 2019 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2018.11.001
Abstract: Worldwide, human norovirus (HuNoV) is a major cause of intestinal infectious gastroenteritis, leading to morbidity and mortality in children and the elderly, and it thus has major social and economic influences. Neither an effective vaccine… read more here.
Keywords: epithelial cells; intestinal epithelial; human induced; human norovirus ... See more keywords
A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.06.017
Abstract: A luciferase immunoprecipitation systems (LIPS) assay was developed to define the antigenic specificity and titer of antibodies directed against human norovirus (HuNoV). Recombinant proteins, expressed by plasmid constructs encoding Renilla luciferase (Ruc) fused to the… read more here.
Keywords: luciferase immunoprecipitation; lips assay; hunov; human norovirus ... See more keywords
Virucidal efficacy of food additive grade calcium hydroxide against surrogate of human norovirus.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.10.017
Abstract: An alkaline agent, namely, food additive grade calcium hydroxide (FdCa(OH)2) in the solution, powder and suspension forms was evaluated as a virucidal agent, using a murine norovirus (MNV) as the surrogate for human norovirus. The… read more here.
Keywords: food additive; grade calcium; norovirus; additive grade ... See more keywords
Fucose-Functionalized Precision Glycomacromolecules Targeting Human Norovirus Capsid Protein.
Sign Up to like & getrecommendations! Published in 2018 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.8b00829
Abstract: Norovirus infection is the major cause of nonbacterial gastroenteritis in humans and has been the subject of numerous studies investigating the virus's biophysical properties and biochemical function with the aim of deriving novel and highly… read more here.
Keywords: precision glycomacromolecules; binding sites; human norovirus; fucose functionalized ... See more keywords