Meiotic defects in human oocytes: Potential causes and clinical implications
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DOI: 10.1002/bies.202200135
Abstract: Meiotic defects cause abnormal chromosome segregation leading to aneuploidy in mammalian oocytes. Chromosome segregation is particularly error‐prone in human oocytes, but the mechanisms behind such errors remain unclear. To explain the frequent chromosome segregation errors,… read more here.
Keywords: chromosome segregation; defects human; meiotic defects; human oocytes ... See more keywords
Profiling of mitochondrial heteroplasmy in single human oocytes by next‐generation sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23655
Abstract: Mitochondrial DNA (mtDNA) plays a crucial role in the development of a competent oocyte. Indeed, mtDNA alterations may predispose to chromosome nondisjunction, resulting in infertility due to a reduced vitality and quality of oocytes and… read more here.
Keywords: human oocytes; single human; mtdna; next generation ... See more keywords
Mitochondrial replacement by genome transfer in human oocytes: Efficacy, concerns, and legality
Sign Up to like & getrecommendations! Published in 2021 at "Reproductive Medicine and Biology"
DOI: 10.1002/rmb2.12356
Abstract: Pathogenic mitochondrial (mt)DNA mutations, which often cause life‐threatening disorders, are maternally inherited via the cytoplasm of oocytes. Mitochondrial replacement therapy (MRT) is expected to prevent second‐generation transmission of mtDNA mutations. However, MRT may affect the… read more here.
Keywords: replacement; mitochondrial replacement; human oocytes; replacement genome ... See more keywords
Bio‐inspired solute enables preservation of human oocytes using minimum volume vitrification
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Tissue Engineering and Regenerative Medicine"
DOI: 10.1002/term.2439
Abstract: The ability to cryopreserve human oocytes has significant potential for fertility preservation. Current cryopreservation methods still suffer from the use of conventional cryoprotectants, such as dimethyl sulphoxide (DMSO), causing loss of viability and function. Such… read more here.
Keywords: vitrification; bio inspired; preservation human; human oocytes ... See more keywords
Study of the mechanical properties of fresh and cryopreserved individual human oocytes
Sign Up to like & getrecommendations! Published in 2019 at "European Biophysics Journal"
DOI: 10.1007/s00249-019-01379-y
Abstract: In assisted reproduction technologies, the cryopreservation of oocytes is a common procedure used to circumvent female infertility. However, some morphological and functional alterations of oocytes have been observed depending on the protocol applied. In this… read more here.
Keywords: freeze thawing; microscopy; human oocytes; individual human ... See more keywords
Amyloid-like substance in mice and human oocytes and embryos
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-019-01530-w
Abstract: PurposeTo identify and characterize amyloid-like substance (ALS) in human and mouse oocytes and preimplantation embryos.MethodsAn experimental prospective pilot study. A total of 252 mouse oocytes and preimplantation embryos and 50 immature and in vitro matured… read more here.
Keywords: like substance; embryos; amyloid like; microscopy ... See more keywords
High-security closed devices are efficient and safe to protect human oocytes from potential risk of viral contamination during vitrification and storage especially in the COVID-19 pandemic
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-021-02062-y
Abstract: The main purpose and research question of the study are to compare the efficacy of high-security closed versus open devices for human oocytes’ vitrification. A prospective randomized study was conducted. A total of 737 patients… read more here.
Keywords: human oocytes; high security; rate; vitrification ... See more keywords
Developmental competence and apoptotic gene expression patterns of mature and immature human oocytes retrieved from controlled ovarian stimulation cycles.
Sign Up to like & getrecommendations! Published in 2018 at "Reproductive biology"
DOI: 10.1016/j.repbio.2017.11.002
Abstract: The purpose was to assess the developmental competence of the in vitro or in vivo matured human oocytes as well as the apoptotic genes expression of cumulus cells (CCs) regarding nuclear maturity status of associated… read more here.
Keywords: oocytes retrieved; expression patterns; human oocytes; developmental competence ... See more keywords
Functional Human Oocytes Generated by Transfer of Polar Body Genomes.
Sign Up to like & getrecommendations! Published in 2017 at "Cell stem cell"
DOI: 10.1016/j.stem.2016.10.001
Abstract: Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer… read more here.
Keywords: oocytes generated; functional human; human oocytes; polar body ... See more keywords
Loss of Centromere Cohesion in Aneuploid Human Oocytes Correlates with Decreased Kinetochore Localization of the Sac Proteins Bub1 and Bubr1
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep44001
Abstract: In human eggs, aneuploidy increases with age and can result in infertility and genetic diseases. Studies in mouse oocytes suggest that reduced centromere cohesion and spindle assembly checkpoint (SAC) activity could be at the origin… read more here.
Keywords: bub1 bubr1; centromere cohesion; human oocytes; sac ... See more keywords
Cytoplasmic maturation in human oocytes: an ultrastructural study †
Sign Up to like & getrecommendations! Published in 2021 at "Biology of Reproduction"
DOI: 10.1093/biolre/ioaa174
Abstract: Abstract Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain… read more here.
Keywords: cytoplasmic maturation; maturation human; human oocytes; egg ... See more keywords