Structural insights into the human PA28–20S proteasome enabled by efficient tagging and purification of endogenous proteins
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DOI: 10.1073/pnas.2207200119
Abstract: Significance We report an approach of combining CRISPR-Cas9 gene editing with florescence cell sorting to tag and purify human endogenous protein complexes from HEK cells for structural studies by single-particle cryogenic electron microscopy. This procedure… read more here.
Keywords: 20s proteasome; human pa28; structural insights; insights human ... See more keywords