In vitro reconstitution of chaperone-mediated human RISC assembly.
Sign Up to like & getrecommendations! Published in 2018 at "RNA"
DOI: 10.1261/rna.063891.117
Abstract: To silence target mRNAs, small RNAs and Argonaute (Ago) proteins need to be assembled into RNA-induced silencing complexes (RISCs). Although the assembly of Drosophila melanogaster RISC was recently reconstituted by Ago2, the Dicer-2/R2D2 heterodimer, and… read more here.
Keywords: risc assembly; reconstitution; human risc; risc ... See more keywords