Optimized use of Oxford Nanopore flowcells for hybrid assemblies
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DOI: 10.1099/mgen.0.000453
Abstract: Hybrid assemblies are highly valuable for studies of Enterobacteriaceae due to their ability to fully resolve the structure of mobile genetic elements, such as plasmids, which are involved in the carriage of clinically important genes… read more here.
Keywords: use oxford; nanopore flowcells; running times; optimized use ... See more keywords
Benchmarking hybrid assembly approaches for genomic analyses of bacterial pathogens using Illumina and Oxford Nanopore sequencing
Sign Up to like & getrecommendations! Published in 2020 at "BMC Genomics"
DOI: 10.1186/s12864-020-07041-8
Abstract: Background We benchmarked the hybrid assembly approaches of MaSuRCA, SPAdes, and Unicycler for bacterial pathogens using Illumina and Oxford Nanopore sequencing by determining genome completeness and accuracy, antimicrobial resistance (AMR), virulence potential, multilocus sequence typing… read more here.
Keywords: quality; hybrid assembly; reference; long reads ... See more keywords